Personal communication from Iswar Hariharan, 24 Jul 2001 [Curator's note: The amino acid affected in the Tsc1[R453X] allele is at position 460, not at position 453 as stated in FBrf0135684. There is an error in the amino acid numbering in figure 3 of FBrf0107789; the 4th row amino acid number should read 468 instead of 461. The coordinate for Tsc1[R453X] in FBrf0135684 was determined by using the figure from FBrf0107789 and counting backwards from 461.] The amino acid affected in the Tsc1[R453X] allele and the source of the error were verified by Dr. Iswar Hariharan in response to a curator query: Date: Tue, 24 Jul 2001 15:34:39 -0400 To: Leyla Bayraktaroglu From: Iswar Hariharan Subject: Re: question about Tsc1 mutation Tsc1[R453X] Hi Leyla: Yes - your explanation is correct. We used the figure from the Ito and Rubin paper and counted back from '461'. Please indicate that the Arg to Stop mutation affects residue 460 and also indicate the mislabeling in the Ito and Rubin paper (otherwise it might cause confusion if somebody tries to find the residue in the figure in the paper using Flybase residue numbers). Thanks and best wishes, Iswar