Subject: Ecol\lacZS138716 Hi Chihiro, I was just looking at the entry in Flybase of our latest paper and realised that the LacZ lines that we used are cited. If you are interested, I have additional information which by the time we published the paper was not available. I rescued the P of the line lacZS138716 and sequenced the flanking genomic sequence, and found out that the insertion is in the 5'UTR of the gene misshapen (msn). It also fails to complement other msn mutant alleles. Cheers, Hernan.- Hernán López-Schier (St Johnston lab) Wellcome/CRC Institute, University of Cambridge Tennis Court Road, CB2 1QR Cambridge; United Kingdom