Subject: FlyBase query arcUAS
Dear Gillian:
You are correct that the arc allele is a replacement of l(2)k11011 with a P
element containing UAS sequences. This particular P element we obtained
from John Merriam, who made it himself. I do not think it has been
published yet. ..
With best regards,
Judith
>Dear Judith,
>
>I was just correcting some of our curation in the FlyBase files, when I
>came across something from your paper:
>
>Liu and Lengyel, 2000, Dev. Biol. 221(2): 419--434
>
>In this paper (on page 428) you describe the 'aUAS' allele
>(<up></up> == superscript).
>
>You state that 'A GAL4 inducible promoter, in which five UAS enhancers
>are fused upstream to the hsp70 basal promoter, was inserted upstream
>of the endogenous arc gene by P element replacement. This allele with
>UAS enhancers upstream of the arc gene was designated aUAS'
>
>From this, I think that this allele was made by replacing the P element
>sequences in 'arcP' (also known as l(2)k11011) with P-element
>sequences which contain UAS sequences (rather than making a UAS-arc
>construct in vitro and then injecting it). Is this correct ? (At the
>moment we have the allele as a UAS-arc construct in our files, but I
>think that we got it wrong when we first curated the paper).
>
>I would be grateful if you could tell me which vector you used to make
>aUAS, i.e. what is the source of the UAS sequences \- is it pUAST, or
>P{EP} (described in Rorth, 1996, Proc. Natl. Acad. Sci. USA. 93(22):
>12418--12422), or is it a different vector ? I need to know this
>information, so that I can record which type of P-element sequences
>(containing UAS) are inserted upstream of the arc gene.
>
>Any information you give me would be recorded as a personal
>communication from you to FlyBase,
>
>I look forward to hearing from you,
>
>Gillian
>
>--------------------------------------------------------------
>Gillian Millburn.
>
>FlyBase (Cambridge),
>--------------------------------------------------------------
Judith Lengyel
Department of Molecular, Cell & Developmental Biology
Box 951606
UCLA
Los Angeles, CA, USA 90095-1606