Subject: Re: FlyBase query
Hi Gillian,
I made the constructs, and the transformants, (in 1983), both of which are
unpublished. The w+ sequence is an EcoR1 fragment from a lambda phage (a
gift of K. Burtis) inserted into a Carnegie vector. I can look up more
details if you wish, but from memory a similar w+ fragment was first shown
to rescue w- in a 1984 Cell paper by Tulle Hazelrigg. I'd also have to
look up which of 'P{wA}' or 'P{C1}' my fragment would correspond to. The Y
insertion was isolated as a secondary P transposase-induced jump.
Please get back to me if there is any more information you might require.
Regards, Allan
At  17:35  31/10/01 \+0000, you wrote:
>Dear Dr. Lohe,
>
>I am curating a paper for FlyBase:
>
>Haller and Woodruff, 2000, Genome 43(2): 285--292
>
>in which they use a Pw+ element on the Y chromosome that you
>isolated. The insertion is called 'Pw+YAL' in the paper. Do you know
>which specific w+ transposon this is a Y-linked insert of \- in the
>methods they say that the P-element carries a 11.7kb w+ sequence \- this
>makes me think that the Pw+ element in this particular insert is
>either 'P{wA}' or 'P{C1}'.
>
>These constructs are described in:
>
>Levis et al., 1985, EMBO J. 4(15A): 3489--3499
>
>and both contain a 11.7kb w+ sequence.
>
>Do you know which transposon your Pw+YAL insertion contains ?
>
>I look forward to hearing from you,
>
>Gillian
>
>--------------------------------------------------------------
>Gillian Millburn.
>
>FlyBase (Cambridge),
>--------------------------------------------------------------
>