Subject: Flanking sequence for P{lacW}G0280a \- Correction
A few minutes ago I sent a message regarding the flanking sequence
for P{lacW}G0280a. The message contained an error in the distance of
this insertion from the 5' end of the SP71 gene mRNA. Here is a
revised version of this communication, in its entirety:
There is a GenBank record ( gi:11071393 ) with the genomic sequence
flanking the P element insertion P{lacW}G0280a (FlyBase ID:
FBti0015480). This is an insertion in 1A5-6, which is one of two
insertions in Bloomington stock 12291. The 5' end of this sequence,
which corresponds to the beginning of the 8 bp target site
duplication flanking the insertion, maps to genomic scaffold segment
AE002567.1 position 26111. This is 90 bp upstream of the 5' end of
the mRNA for the SP71 gene, according to the submission by Selano et
al. (GenBank accession AF212322.1).
\--
\------------------------------------
Robert W. Levis, Ph.D.
Carnegie Institution of Washington
\------------------------------------