Subject: query about Mcm6 paper
Hi Brian,
I have now finished curating your MBC paper;
Schwed et al., 2002, Molec. Biol. Cell 13(2): 607--620
and I have a a question about the deletions in 6C made by P-element
excision. You used 2 EP elements to make deletions: EP(X)1364 and
EP(X)1445. From looking at Figure 2. I think that:
a. Df(1)6C-190, Df(1)6C-25 and Df(1)6C-40 were made by excisions from
EP(X)1445
b. Df(1)6C-51 was made by an excision from EP(X)1364
could you confirm if this is correct.
Also, what EP element were each of the following derived from:
a. Df(1)6C-310
b. 6C-166
c. 6C-157
I will curate your reply as a personal communication to FlyBase, so
people can see how we know this information,
thanks,
Gillian
Subject: Re: query about Mcm6 paper
>Hi Brian,
>
>I have now finished curating your MBC paper;
>
>Schwed et al., 2002, Molec. Biol. Cell 13(2): 607--620
>
>and I have a a question about the deletions in 6C made by P-element
>excision. You used 2 EP elements to make deletions: EP(X)1364 and
>EP(X)1445. From looking at Figure 2. I think that:
>
>a. Df(1)6C-190, Df(1)6C-25 and Df(1)6C-40 were made by excisions from
>EP(X)1445
>
>b. Df(1)6C-51 was made by an excision from EP(X)1364
>
>could you confirm if this is correct.
\------------Yes that is correct.
>
>Also, what EP element were each of the following derived from:
>
>a. Df(1)6C-310 : EP(X)1364
>
For the next two, the information on the starting P is noted below.
However, since these events complemented MCM6, and since we did not find a
deletion by Southern, all we really know is that these are X linked
lethals. ..
b. 6C-166- EP(X)1445
>c. 6C-157- EP(X)1364
>
>I will curate your reply as a personal communication to FlyBase, so
>people can see how we know this information,
>
>thanks,
>
>Gillian
Brian R. Calvi, Ph.D.
Assistant Professor
Department of Genetics
University of Pennsylvania
School of Medicine
415 Curie Blvd., CRB rm 452A
Philadelphia, PA 19104-6145