Subject: Revised cytology of Df(3R)23D1 I reanalyzed the polytene cytology of Df(3R)23D1 and found the breakpoints to be 94A3-4;94D1-4. The deletion removes most of 94D1-4, leaving only a thin portion from the distal edge of the quadruplet. The 94A3,4 doublet is nearly intact.