Subject: Helping FlyBase: CSH-205 Dear Greg, We are currently curating the abstracts for the 2001 CSH Neurobiology of Drosophila Conference, for FlyBase. I am writing in connection with your abstract: 'A novel Dbl family Rho-GEF promotes axon attraction to the cns midline and overcomes robo repulsion.' You mention a gene that is new to FlyBase, GEF64C. Do you know which of the Genome Project CG annotations your gene corresponds to? Might it be CG13709? All the CGs have corresponding gene records in FlyBase already and we don't like to make duplicate records for what is actually the same gene unless we can't avoid it. We would of course rename CG13709 if it is your GEF64C. Thank you very much for your help, with best wishes, Rachel. \---------------------------------------------------------------------- Rachel Drysdale, Ph.D. FlyBase (Cambridge), \---------------------------------------------------------------------- Subject: Re: Helping FlyBase: CSH-205 Hi Rachel, GEF64C cDNAs appear to be comprised of two different CG#s I'm not sure what this means, but we had the same experience with the highwire gene. The business end (i.e. the part with the Dbl GEF domain) corresponds to the CG13709 as you indicated in your e-mail. The N- teminus corresponds to the neighbouring CG13710 Best Greg \-- Greg J. Bashaw Ph.D. Assistant Professor Department of Neuroscience University of Pennsylvania School of Medicine 1113 BRBII/III 421 Curie Blvd. Philadelphia, PA 19104