Subject: Helping FlyBase: ADRC-10768
Dear Susan,
We are currently curating the abstracts for the upcoming 43rd
(San Diego) Annual Drosophila Research Conference, for FlyBase.
I am writing in connection with your abstract:
'ed and fred: inhibitors of EGF receptor signaling.'
You mention a gene that is new to FlyBase, fred, and say that it lies
just upstream of ed. Having checked upstream of ed in Geneseen I see
nothing very much (though this depends on exactly what you mean by
'just upstream'). Could you confirm for me (or otherwise set me
straight) that fred was missed in the genome annotation project? If it
was I urge that you submit a sequence accession record for fred to
Genbank so that it will then be drawn onto the genome annotation (there
is a data path within FlyBase that will ensure that this is so), next
time the annotation looks at that region of the chromosome.
Thank you very much for your help,
with best wishes,
Rachel.
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Rachel Drysdale, Ph.D.
FlyBase (Cambridge),
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Subject: Re: Helping FlyBase: ADRC-10768
Dear Rachel,
Thanks for your note. Several predicted genes (CG12675, CG3393, CG3390)
match pieces of Fred, but none matches the 5' end of the protein. The 5'
end of Fred is at 255,363 of AE003578; echinoid's 5' end is at 295,068, and
they are divergently transcribed. I guess 40,000 bases away may not be
'just upstream', but then again, each of these genes spans more than 80,000
bases (with many introns, of course), so their 5' ends are relatively close
together!
I'll submit the fred sequence to genbank ASAP. Good luck with your annotation.
Susan