Subject: Helping FlyBase: ADRC-10572
Dear Marcin,
We are currently curating the abstracts for the upcoming 43rd
(San Diego) Annual Drosophila Research Conference, for FlyBase.
I am writing in connection with your abstract:
'The Drosophila rad50 mutants are pupal lethal, however the third
instar larvae show elevated levels of anaphase bridges in dividing
cells.'
You mention a gene that is new to FlyBase, rad50. Do you know which of
the Genome Project CG annotations your gene corresponds to? Is it by
any chance CG6339? All the CGs have corresponding gene records in
FlyBase already and we don't like to make duplicate records for what is
actually the same gene unless we can't avoid it. If your gene does not
correspond to a CG then perhaps you could tell me its map location, as
this is valuable information for the genome annotation project.
You also mention a P{EP} insertion mutant. Is this one of the public
collection of EP lines? If so I would be most grateful if you could
tell me the insertion identifier.
Thank you very much for your help,
with best wishes,
Rachel.
\----------------------------------------------------------------------
Rachel Drysdale, Ph.D.
FlyBase (Cambridge),
\----------------------------------------------------------------------
Subject: Re: Helping FlyBase: ADRC-10572
Dear Rachel,
It appears that gene CG6339 encodes a protein that shows high homology
to yeast and human Rad50 proteins involved in DNA double strand break
repair and the following information was obtained from the FlyBase. The
map positions of CG6339 as well as the EP(2)2600 insertion were also
from the FlyBase. In addition to the information provided by the FlyBase,
we confirmed the exon-intron structure of the CG6339 gene by sequencing
the SD05424 EST clone as well as the 5' 1.5 Kb cDNA fragment obtained
from the RT-PCR reaction.
Furthermore, we utilized EP2600 insertion inserted roughly 750bp upstream
of the CG6339 to perform P-mediated mutagenesis. We identified one insertion
into the third exon of the CG6339. The phenotype of Dmrad50EP1 mutant flies
is described in the abstract the flies are still in our collection.
Sincerely,
Marcin