Gene or accession: CG7462
Release: 2
Missed gene
Gene cDNA sequence:
>ANTTTTGGAGTTGAAACATNGGAAGGAAAACGGGCAGCAAAACNGAAAAATTGTTCCTTCGAGAACCGTGAACTTAAT
AAGGGACTGTGTGAGAGCAGCCAGGAGTCCAATCAGACACATACACACACACAAAGACATTTACACCCATACACTGACA
CTGACACGCACACAACCGAATCGGTCGCCTGTACGAACACACCCTCTCACACACTCATGCACTCGTGTACATATAGCTT
ACATAAAGTCTAAGGAAAAATCGATAGGAGTGCATTAATAATTCAGCGAAAACGAAACGAGCATAAGGTCTCGAGCAAA
AATCTTCAAGTAGTGCGAGTTTTCCGCCTCTGCTAACGGTGAAGAAAAGTTCCAGGCCTCGTGAAAAAATATGACTAAT
CTGCAGTGACGACATACGCGCCGCTTACACAATTTCTCTACACCAATTAACCGCTGAAATCAATTCAAGACGGGCATTT
TACCATTGGCAATTGTGAAAAAATGGTCACCGAAAATGGAGCACAGGGCGATGGAAACACTTCCTTCCTGCGTGCCGCT
CGCGCCGGAAATCTGGAGCGAGTGCTCGAGCATTTGAAGAACAACATTGACATTAACACCAGCAATGCGAATGGCTTGA
ATGCCCTGCACCTGGCCTCCAAGGATGGCCACATCCACGTCGTCTCGGAACTCCTTCGTAGGGGCGCGATTGTGGATAG
TGCCACCAAAAAGGGAAACACTGCCCTCCACATCGCCTCATTGGCTGGACAGGAGGAGGTGGTGAAGCTACTGCTGGAG
CACAATGCCTCCGTGAATGTGCAATCCCAGAATGGATTCACTCCACTCTACATGGCTGCCCAGGAGAACCACGATGCTG
TGGTGCGTCTCCTTTTGTCCAACGGAGCCAACCAGAGTCTGGCCACCGAGGATGGCTTCACCCCACTGGCGGTGGCCAT
GCAGCAGGGTCATGACAAAGTCGTTGCTGTCCTCCTCGAGAGT!
GATACTCGTGGAAAGGTCAGGCTTCCTGCGCTGCACATTGCCGCCAAAAAGGACGACGTCAAGGCAGCGACTCTGCTTC
TCGATAATGACCATAATCCGGACGTGACTTCCAAGTCGGGCTTCACCCCGCTGCACATTGCTTCCCATTATGGTAACCA
AAACATCGCCAACCTACTCATCCAGAAGGGCGCCGATGTCAACTACTCGGCCAAGCACAACATCAGTCCGCTGCATGTG
GCTGCTAAGTGGGGTAAGACCAACATGGTTTCCCTGCTTTTGGAAAAAGGTGGTAACATCGAGGCAAAGACCCGGGACG
GACTTACTCCGCTCCATTGCGCCGCTCGCTCGGGTCATGAGCAAGTTGTTGACATGCTACTCGAACGAGGAGCTCCCAT
CTCTGCCAAGACCAAAAATGGTTTGGCACCCCTCCATATGGCCGCCCAGGGTGAGCATGTTGATGCCGCCCGCATCCTC
TTGTACCATCGTGCTCCCGTCGACGAGGTGACAGTGGATTATCTCACTGCTCTTCATGTGGCTGCTCACTGTGGTCATG
TTAGGGTGGCTAAGCTTTTACTAGATCGGAATGCTGATGCCAATGCAAGGGCTTTGAATGGATTCACTCCACTGCACAT
TGCCTGCAAGAAGAACCGCTTGAAGGTGGTAGAGTTGCTCTTGCGACATGGTGCCAGCATTAGTGCCACCACAGAGAGT
GGACTTACTCCGCTCCATGTGGCCGCCTTTATGGGCTGCATGAACATTGTCATCTATCTGCTGCAGCACGATGCCAGTC
CCGATGTGCCCACTGTGCGCGGAGAAACGCCACTCCATTTGGCCGCCAGAGCCAACCAGACCGACATCATTCGCATCCT
GCTGCGCAATGGCGCCCAGGTGGACGCTCGTGCCCGAGAGCAACAGACACCGCTGCACATCGCTTCGCGACTGGGTAAT
GTAGACATCGTGATGCTTCTGCTGCAACATGGCGCCCAAGTGG!
ACGCAACCACCAAGGACATGTACACGGCACTTCACATCGCTGCCAAGGAGGGCCAGGAT!
GAGGTGGCCGCTGTACTAATTGAGAACGGCGCCGCCTTGGATGCCGCCACCAAGAAGGGATTCACTCCACTTCACTTGA
CGGCCAAATATGGACACATTAAGGTTGCCCAGTTGCTGCTCCAAAAGGAGGCAGATGTGGATGCGCAGGGCAAGAACGG
TGTCACCCCGTTGCATGTGGCTTGCCACTACAATAACCAGCAGGTTGCCTTGTTGCTTTTGGAGAAGGGAGCAAGTCCA
CATGCCACGGCCAAGAATGGACATACCCCGCTCCATATTGCCGCAAGGAAGAACCAGATGGACATAGCTACTACTCTAT
TGGAATATGGCGCTTTGGCCAATGCCGAGAGCAAAGCCGGATTTACTCCACTCCATTTGAGCAGCCAGGAGGGTCATGC
CGAGATCTCCAATCTGTTGATCGAGCACAAGGCCGCCGTCAATCATCCGGCTAAGAATGGTCTAACTCCCATGCATCTG
TGTGCCCAGGAGGATAATGTGAATGTGGCTGAGATTCTGGAGAAGAACGGCGCCAATATCGACATGGCCACCAAGGCAG
GCTACACTCCCTTGCATGTGGCCTCTCACTTTGGACAGGCCAACATGGTGCGTTTCTTGCTGCAGAATGGCGCCAATGT
GGATGCGGCCACCTCAATTGGCTATACTCCGCTCCATCAGACCGCCCAGCAGGGTCATTGTCATATTGTGAATCTCCTG
CTGGAGCACAAGGCTAATGCCAACGCTCAGACGGTCAATGGACAGACGCCTTTGCACATTGCCCGCAAACTGGGCTACA
TAAGTGTCCTAGACTCGTTGAAAACCATCACCAAGGAGGACGAAACAGCTGCTGCTCCTTCCCAAGCCGAGGAGAAGTA
TCGCGTGGTGGCTCCCGAGGCCATGCACGAATCCTTCATGTCCGACTCCGAGGAAGAGGGCGGCGAAGACAATATGCTA
TCAGATCAACCATACCGCTATTTGACCGTTGATGAAATGAAAT!
CCCTAGGCGACGACTCGCTGCCCATCGATGTGACCCGTGATGAGCGCATGGACTCCAACCGGATGACCCAGAGCGCCGA
GTATGCCTCTGGTGTTCCGCCCACCATTGGGGAGGAGGTGATCAGTCCCCACAAGACCCAAGTCTACGGCAGTTCACCC
AAGGCCACCGTGGATGGAGTGTACATTGCCAATGGATCTGGTCACGATGAGCCGCCGCATGTGGGTCGCAAGTTAAGCT
GGAAGAGCTTCTTGGTCTCCTTCCTGGTGGATGCCCGTGGAGGAGCAATGCGCGGCTGTCGGCACAGTGGCGTTCGCAT
GATCATCCCTTCGAGGTCCACTTGTCAGCCAACCAGGGTAACCTGTCGTTATGTAAAGCCGCAGCGCACAATGCATCCG
CCCCAGTTGATGGAGGGCGAGGCTTTGGCCAGTCGTGTCCTGGAACTGGGTCCTTGCTCCACCAAGTTTATTGGGCCAG
TGGTCATGGAGGTGCCACACTTTGCATCGCTGCGTGGCAAGGAGCGCGAGATCATAATATTGCGATCGGACAATGGAGA
AACATGGCGGGAGCACACCATCGATAACTCCGAGGAGATCATACACGATGTCCTGCAGCAGTGCTTTGAACCAGAGGAG
ATTGCCCAACTGGAGGAGCAGGCTGGCAACCATGTCTGCCGCTTTGTCACCTACGACTTCCCTCAGTACTTTGCCGTCG
TATCGCGCATACGCCAGGAGGTCCATGCCATTGGACCGGAGGGCGGCATGGTGTCAAGCACCGTGGTGCCACAGGTGCA
GGCCGTCTTCCCACAGGGAGCCCTCACCAAGAAGATCAAAGTTGGCTTACAGGCCCAACCGGTTGATCCCGATCTCACG
GCGAAGCTTTTGGGTCGCGGCGTGGCCGTATCCCCAATTGTTACGGTCGAGCCGCGTCGCCGTAAATTCCACAAGGCCA
TCACCCTCAGCATGCCCGCCCCCAAGGCTCACGGCCAGGGTAT!
GATTAACCAGTACTCGGGCAATACGCCCACCCTGCGTCTGCTATGCTCCATTACAGGCG!
GACCATCTCGTGCTCAATGGGAAGATGTCACCGGTTCCACGCCCTTGACATTTGTCAATGACTGCGTCTCCTTTACGAC
CACAGTATCAGCTCGTTTCTGGCTGATGGATTGCCGCAACATTTCGGATGCCACCAAAATGGCAACTGAACTGTACAAG
GAAGTCATCCATGTGCCGTTTATTGCCAAGTTTGTGGTATTCGCCAAGAAGGTAGAACCATTCGAAGCAAGACTCCGAG
TCTTTTGCATGACCGACGATCGTGAGGACAAAACTCTGGAGAAGCATGAGCTCTACACAGAAGTGGCCAAGAGCCGCGA
TGTCGAGGTCCTCGAAGGCAAGCCGCAGTACATCGAGATGGCTGGCAATTTGGTGCCAGTGACCAAATCTGGCGACCAG
CTCCAGGTGCAATTCAAGGCCTTCCGAGAGAACCGCCTACCGTTCACAGTACGAGTCAAGGATCAACATGCCGACATTG
TTGGACGCACTTTGTTCATGAAGGAACCAAAGGTAGCCAAGGGCGAGCCACCGCAACAGCCCATCTGCATCCTGAACAT
CGTTCTTCCGGAAGCTGTAATTCCCGATTCCACGACCGCCTTTTCGGACCGCGTCACCTCCGCCTACAGGACCTCAATG
TTCAGCTTGAGCAAGCATCAAAATGACCATTACATTGGCGACATCCGCATCGTCGATCTATCCAACCTGTTGGGTAAGG
ATTGGATTCAGCTGGCCCCGGAGATTGGCATCAATGGAGAGGAGATCGATGAGATCATCAACCAAAACACAGATAGCAT
TGCCAGGCAGGCGCAGAGCATGATTCGCCTGTATAAGGACAAACCTAACTACGATATTCTTTCGCTGGAAACTGCTCTC
AAAAACATTGGACGAGACGACATCATGAAAAAGTGCAAGAGTGGACGACTATCGCATTCGCGTGAATTCGATGAGGCGG
ACCTTATGAAGAACTCGGAGTCCGTGGAGGAACTGGTTCGCAG!
GGAAAGCAAGCGAATCCAGCAAATTAACGAACGCGAAGAAGTCAAATACTCTGCCGAGGAGAAGGAGGTCGAGGAATCG
GAGTCTGATGAAGAAGCTGCCAAGCGAACCGTAGCCGAACGGCGGGAAAAGATTGTTAAGCGTCTGTCCATCGAACGAT
CAATTCCAGCCTCCACTCAGAAAAAAGAGATCACCCGCGAAATAACCGAAATCAAGCGTAAGAGTCTAATCGAGGACAA
GAAGGCTCACCATGAGTCCGAAATTCTTATGCAACTGCCTGCGGATAACGTGATTATCAAGACAACAACTGTTCCCGAC
CAAGTCATTAAGATGAAGATGGGCAAGATGGACTCAACCGAGGTGAGCAAGAGCGAGTTCGACAAGGAACTTACGCACA
AGTTCAAGACATCAGGGCGTAGCTCAGAGGAGGAAGACCAGCCATCGTCTCCAGATCAGACTGACAAGATTGTCCAGGA
CATTAGTGCTGCCGAAAAGAAGGAGAAGGATGGCGTTACCTTCAGCCGAGTGACGACAATAACCCGCCAAGAGGCCCGC
GACATTACCGAAGACTTCTTAGAGATTGAGAAGCGCAGCCAGCTCCCGGCGACTTCCACAACCGCAACAGTTCATGAGA
AGTTCGTTGAGGAGATCAAGGAGAAGACCAGCCCTCTGGCTTCAGTGCCACAAGAAACAGTCAAGGAGGTGCAACAAGT
CATCAGCGAGGTGACCGAAATAGCCAGCAAGAAGGTTGAAAACATTATTAGCTCCTTTGAGAGTAGCAAGTCTGTGGAT
GCTACTACAGTTCTGCCCACACAGCCGTCTGTTGAATCAACGAAGGTTAGCGAGACCATTAAGAACCTCGAGGATGCCA
AGGCCGTGTCTGCGGAACAAGTGAAAACTGTCCATGTCGTTGAGAGCTCTAGTATCGAGGAGACTATTGCCGAATTCGA
AGCCAAGAAGGTTAAATATGACTTCCATGGCGGTGAGCCCAAG!
ACACAGATCCCAAAGTTTACGCGGAAACCAAGCGATGACTCTATGAAGCCCACCGCAGC!
TCCACGTGCTACAGTTGAATCCGAAACTGAATCGGTCATTGAGACTAAGGCCGAAAAACCGATCTCAAAGATACCAGTA
AAGACAATACCAACAGAAGCTCAGAAGGTTAGCGAAGTAGATGCACGTAAGATTACTCAAGACTTCCTCCAGGGTGAGA
AACTGGCGGCCGAACCCAAGCCATCGCCAGCAGCCAGCAAGATACCAAAGGTGGAGCCGAGGAAATCTGTTGATAAGCA
GGTCGATCGGGAATCAAAAATCTTGGATGATGTTGTGGCCTCTACGGCCACCATTATGACCGCCGGACTGGGAGATGAG
CAGCTCAAGGATCAACTCGTCGATCACTCAGAAATTATCGCCAAGTCCGAAACAGTCGCTGAAAAGGTAACCGAACTGC
TTGACACATTCCACAAGATCGAGGAGAAGGTCACCAAATCTAAAAAGACCTCGGAAATATCGAGCAAAGTGGAGGAACT
GGTTAAAATCGAGGAAAAACCTCTCTCACAAGTTCAACCCAAGGAGGACAAGGTGGCGGAAAAGGTTGCTGAAGTTATC
GAAACATTCCACAAAATCGAGGAGCAGATCACCACTGCCGATGCCCGCGAGCTAACTCGTGATTTCCTGAGCACGGAAC
AACGAAACCAATTGCCGAGCATGCCACAAGCTGCTGAGAAGCCCCTTGAATCTAGCTTGACATCCACCAGTGCCTCGGA
ACCAGAATCAATAGTCACAGTGAAGCCATCACCCCCAGCGAGTAAGATACCTGTAGTGGAACCCAGGAAAATCGTTTTC
GACGAATCTACAAAGCCTCTGATCGAGCCTGAGCCAGTGAAGACCACTGAAAAGAAGCCTCTTAATGAGCGACAACTAA
CCGCTGATTTCTTGAGCATGGAGCAACAAACCCAGCTGGTCTCTGAGCCTGCCAAATCGTTGGTGGAAGAGGTGATGAA
GTCCGCCGAACAAATGGTGGAGCAACCGAAACAGCAAAAATCC!
CTGAATGAGCGACAACTTACAGAGGACTTTCTGCTTATGGAACAGCAGACACAACTGCATTCAGACATTGTTAAGCCAA
CGGATAAGCTGATCGATGGTATTGAGTCCGCAGCGCCGGTTGGAGATGAAGCATCACCGTTCCACACGCCCAAGCTGAC
CACCAGTGTGGTGACACAGGAGTCACAGCACCTGGCGAGTGAATATGACAGTGACACTTTCGGCAAACAGGCCACCATA
CCGTTAGGAGACTCAAAGATTGATCAGGGACTCACTGCACCAGTATCCATGGAACCACGAAAGTCACTGACCGATGCCG
AGTTCTGCAAATCGGTGGGCGAAACAATCACCAAGAAGATGAGTGTGGGAGTTATTGAAATAAGTGATGAGCTCAAGAA
AATTGAGAGCGAAATTCCACATTCCCAGACTCCTCCGCCAACACCGAGTGACAACAAAACCGACAAGCAGAACGAAGAG
CCAGAACTGATCAGCTTGGAACGTGATTACCTGGCCGACACTGTGACCACCCTTCACACAACTACAGAGTCGACCCTCG
AACGCGTTTCTGCCATTACTAAAATTGGTAAGTGCAAGTCCTGA
Protein sequence:
>MVTENGAQGDGNTSFLRAARAGNLERVLEHLKNNIDINTSNANGLNALHLASKDGHIHVVSELLRRGAIVDSATKKGN
TALHIASLAGQEEVVKLLLEHNASVNVQSQNGFTPLYMAAQENHDAVVRLLLSNGANQSLATEDGFTPLAVAMQQGHDK
VVAVLLESDTRGKVRLPALHIAAKKDDVKAATLLLDNDHNPDVTSKSGFTPLHIASHYGNQNIANLLIQKGADVNYSAK
HNISPLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGE
HVDAARILLYHRAPVDEVTVDYLTALHVAAHCGHVRVAKLLLDRNADANARALNGFTPLHIACKKNRLKVVELLLRHGA
SISATTESGLTPLHVAAFMGCMNIVIYLLQHDASPDVPTVRGETPLHLAARANQTDIIRILLRNGAQVDARAREQQTPL
HIASRLGNVDIVMLLLQHGAQVDATTKDMYTALHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQ
LLLQKEADVDAQGKNGVTPLHVACHYNNQQVALLLLEKGASPHATAKNGHTPLHIAARKNQMDIATTLLEYGALANAES
KAGFTPLHLSSQEGHAEISNLLIEHKAAVNHPAKNGLTPMHLCAQEDNVNVAEILEKNGANIDMATKAGYTPLHVASHF
GQANMVRFLLQNGANVDAATSIGYTPLHQTAQQGHCHIVNLLLEHKANANAQTVNGQTPLHIARKLGYISVLDSLKTIT
KEDETAAAPSQAEEKYRVVAPEAMHESFMSDSEEEGGEDNMLSDQPYRYLTVDEMKSLGDDSLPIDVTRDERMDSNRMT
QSAEYASGVPPTIGEEVISPHKTQVYGSSPKATVDGVYIANGSGHDEPPHVGRKLSWKSFLVSFLVDARGGAMRGCRHS
GVRMIIPSRSTCQPTRVTCRYVKPQRTMHPPQLMEGEALASRV!
LELGPCSTKFIGPVVMEVPHFASLRGKEREIIILRSDNGETWREHTIDNSEEIIHDVLQQCFEPEEIAQLEEQAGNHVC
RFVTYDFPQYFAVVSRIRQEVHAIGPEGGMVSSTVVPQVQAVFPQGALTKKIKVGLQAQPVDPDLTAKLLGRGVAVSPI
VTVEPRRRKFHKAITLSMPAPKAHGQGMINQYSGNTPTLRLLCSITGGPSRAQWEDVTGSTPLTFVNDCVSFTTTVSAR
FWLMDCRNISDATKMATELYKEVIHVPFIAKFVVFAKKVEPFEARLRVFCMTDDREDKTLEKHELYTEVAKSRDVEVLE
GKPQYIEMAGNLVPVTKSGDQLQVQFKAFRENRLPFTVRVKDQHADIVGRTLFMKEPKVAKGEPPQQPICILNIVLPEA
VIPDSTTAFSDRVTSAYRTSMFSLSKHQNDHYIGDIRIVDLSNLLGKDWIQLAPEIGINGEEIDEIINQNTDSIARQAQ
SMIRLYKDKPNYDILSLETALKNIGRDDIMKKCKSGRLSHSREFDEADLMKNSESVEELVRRESKRIQQINEREEVKYS
AEEKEVEESESDEEAAKRTVAERREKIVKRLSIERSIPASTQKKEITREITEIKRKSLIEDKKAHHESEILMQLPADNV
IIKTTTVPDQVIKMKMGKMDSTEVSKSEFDKELTHKFKTSGRSSEEEDQPSSPDQTDKIVQDISAAEKKEKDGVTFSRV
TTITRQEARDITEDFLEIEKRSQLPATSTTATVHEKFVEEIKEKTSPLASVPQETVKEVQQVISEVTEIASKKVENIIS
SFESSKSVDATTVLPTQPSVESTKVSETIKNLEDAKAVSAEQVKTVHVVESSSIEETIAEFEAKKVKYDFHGGEPKTQI
PKFTRKPSDDSMKPTAAPRATVESETESVIETKAEKPISKIPVKTIPTEAQKVSEVDARKITQDFLQGEKLAAEPKPSP
AASKIPKVEPRKSVDKQVDRESKILDDVVASTATIMTAGLGDE!
QLKDQLVDHSEIIAKSETVAEKVTELLDTFHKIEEKVTKSKKTSEISSKVEELVKIEEK!
PLSQVQPKEDKVAEKVAEVIETFHKIEEQITTADARELTRDFLSTEQRNQLPSMPQAAEKPLESSLTSTSASEPESIVT
VKPSPPASKIPVVEPRKIVFDESTKPLIEPEPVKTTEKKPLNERQLTADFLSMEQQTQLVSEPAKSLVEEVMKSAEQMV
EQPKQQKSLNERQLTEDFLLMEQQTQLHSDIVKPTDKLIDGIESAAPVGDEASPFHTPKLTTSVVTQESQHLASEYDSD
TFGKQATIPLGDSKIDQGLTAPVSMEPRKSLTDAEFCKSVGETITKKMSVGVIEISDELKKIESEIPHSQTPPPTPSDN
KTDKQNEEPELISLERDYLADTVTTLHTTTESTLERVSAITKIGKCKS
Comments: We determined that there are at least 5 different transcripts
(labeled a, b, c, d, and e) from the Ank2 gene (CG7462). There results are in
press:
M. Hortsch, K.L. Paisley, M.Z. Tian, M. Qian, M. Bouley, and R. Chandler: The
Axonal Localization of Drosophila Ankyrin 2 Protein Isoforms Is Essential for
Neuronal Functionality. Mol. Cell. Neurosci. (in press)
Transcript a is shorter than all the other transcripts, encodes a polypeptide
of 1159 aa and is identical to the ORF that was reported in our previous paper
(Bouley et al. 2000).
A differential splice donor site in exon 9 generates the longer transcripts b,
c, d and e that encode polypeptides of 2405, 2386, 2451 and 2465 aa
respectively. These transcripts differ from each other by the differential
splicing of the small exons 13, 14 and 15 at the 3' end of the large ORF.
RT-PCR analysis indicates the transcript b ist the most abundant transcript of
the 4 long transcripts. Above I have pasted the cDNA and protein sequences of
the long b transcript. I will send you the long transcripts and ORF for the
other long Ank2 transcripts c, d and e separately. You may want to substitute
these for the long CG7462 ORF that was predicted by the genome project.
Please send me an email if you need further explanations or information.
With the best regards
Michael Hortsch