Gene or accession: CG18338
Release: 2
Gene annotation error
Genes CG18338 and CG13219 should be merged.
Gene cDNA sequence:
>skiff cDNA
CCGTCGTGTGCAGTCGCTCTCGCTCCCGTTATTCGCCGTTCTGGGATTCCCGCTGGCGGACTCTGGTGGCCATCACCTT
GATAATCCTTATTGTGAAATATGTGAAGTGCTGCAAAGAGTCTAAGGATATCCTGATATATTAACGGTTAACTAAACTA
GTGCGCAATATTAGAGGCCTAAAAATAGCGAAAATAGCAGACATGCCGTGTTTTTGTGCCTCTGTGTGTGCGTGAGTGT
CTGCGAGTGAAGTGGAAATTTAAAAATAAAACAACGCTGGGACTCAACCGAAATCCGTCTGTATCGTATTGGCCAACTG
CCCAGAAAAACTCCAGGAACAGCGCCAAAGAATAACTTGTAAAGGATAAGCAAGGACAGTGACTCGTCGACTGTTGTGT
GACATGCTAGAAGCAGTTATGGCATCGGACATCAACTGGGATCCTGCTCTTTCCAAGCTGATCAGCACGCTCAAGGAGT
CGGAGAATCTATCCAATGACCAGGAAATCGACTTCCTGAAGGCCTTGCTTGAATCCAAGGAACTAAACGCGCTGGTCAA
TGTCCACACGAAGGTGGCCAAAGTGGGTCGAGATGATCGTCTGGCCCCGGTGCTCTCCACCTCTGCCCAGGTGTTGTAC
GAAGTCCTGGAGCAGCTGTCCCAGCATTGTCATCTGAACGATGACTGCAAGGAGGCCTTTCACCTGCTGCAGGACTCTC
ACCTGCAGCACCTTCTGTTCGCCCACGATGCGATTGCCCAGAAGGACTTCTACCCGCACCTGCCGGAGGCGCCTGTCGA
AATGGACGAGGACGAGGAGACCATCAAGATTGTGCAGCTCGTCAAGAGCAACGAGCCCCTGACAGGAGCACAGAGTACG
GAGCCAATTGTTGGCGCCACCATTAAAACGGACGAGGAGTCGGGCAAGATAATCATCGCCAGAATTATGCACGGCGGCG
CTGCAGATCGCTCCGGATTGATTCACGTCGGCGACGAGGTC!
ATCGAGGTCAACAACATCAACGTGGAGGGCAAGACGCCGGGCGATGTGCTCACCATACTGCAAAACTCGGAGGGCACCA
TTACCTTTAAGCTGGTTCCCGCGGACAACAAGGGCGCCCAGCGCGAGTCCAAAGTGCGGGTGAGGGCCCACTTTGACTA
CAATCCGGATGTGGATCCCTACATTCCGTGCAAGGAGGCGGGTCTGGCCTTCCAGCGCGGCGATGTGTTGCACATTGTG
GCCCAAGACGATGCCTACTGGTGGCAGGCGCGCAAGGAGCACGAGCGCTCGGCAAGAGCCGGTTTGATTCCCAGCCGGG
CGCTGCAGGAGCGCCGTATCCTCCACGACCGTACACAGAAAAACGGCACCGATCTGGACTCGAAGCAGAACGACGACTT
TGACCGCGAGCAGATCGCCACGTACGAGGAGGTGGCCAAGTTGTATCCACGGCCCGGAGTCTTCAGGCCCATAGTGCTC
ATCGGAGCACCCGGCGTGGGGCGGAATGAATTGCGCCGCCGGCTGATAGCCCGGGATCCCGAGAAGTTCCGCAGTCCGG
TTCCGTACACCACCCGACCAATGCGCACAGGTGAAGTGGCCGGACGAGAGTACATCTTTGTGGCCCGCGAGAAAATGGA
CGCGGACATCGAGGCGGGAAAGTTTGTGGAGCACGGCGAGTACAAGGGCCATTTGTACGGAACATCGGCGGAGAGTGTC
AAGTCGATTGTGAATGCCGGCTGCGTATGTGTGCTGAGTCCGCACTACCAGGCGATCAAGACTCTGCGCACAGCTCAGC
TGAAGCCCTTCCTCATCCATGTGAAGCCACCAGAGTTGGACATCCTGAAGGCTACGCGAACGGAGGCGCGTGCCAAATC
CACATTCGACGAGGCGAATGCCAGAAGTTTTACGGACGAAGAGTTCGAGGACATGATCAAGTCGGCCGAACGCATAGAT
TTCCTATACGGACACTTCTTCGACGTAGAGCTGGTCAACGG!
CGAACTGGTCAATGCTTTCGAGCAGCTGGTCCAGAATGTCCAGCGACTGGAGAACGAACCGGT!
ATGGGCACCATCCATGTGGGTGCAGTAGATCGGTTGCCAAATGTGGACAAGCATTTCATGCAGTTTGTTGATGAACACC
AAAGTATTTTTGCCATACGAATTTAGCAATCTGCTAGAGAAACGGAGAAGAGACACTATTTACCCGTAGGCGGTCCTGC
TGGGCACTCCCGTCCGGCCTTAGTCCTTTTCACGACATGTACCTTTTTGCGTAACAGTGAAAACAATGTAATGAATACA
ATAATTATTGACAGAAAGTGAAGGAAATTTGAACTCAACCAGCATCTAACTTACAATTAAATTATATAAACTTGTTGTA
CAAAATTTATTTGTGTTCAAAGAATGACTTAATTATAAATGTATGTATTACACGTTGTTGAAATCAAAGTACAGAGTTA
ATCCCCCCGTAAAACCTAAAGCTAATCGTATTGTATCTCAGTTGTATATACACTCACTAACTGAAGCATATTGGATACA
ATGTGAAGTTCTGTGTGACGACATGTCTCATTTCGAAAACTGATTTCACCAAAAACGTAGTGACAAATTGTTGAAAACA
AGTGAGAAACTAATAAAAAAAAAAAA
Protein sequence:
>Skiff protein
MLEAVMASDINWDPALSKLISTLKESENLSNDQEIDFLKALLES
KELNALVNVHTKVAKVGRDDRLAPVLSTSAQVLYEVLEQLSQHCHLNDDCKEAFHLLQ
DSHLQHLLFAHDAIAQKDFYPHLPEAPVEMDEDEETIKIVQLVKSNEPLTGAQSTEPI
VGATIKTDEESGKIIIARIMHGGAADRSGLIHVGDEVIEVNNINVEGKTPGDVLTILQ
NSEGTITFKLVPADNKGAQRESKVRVRAHFDYNPDVDPYIPCKEAGLAFQRGDVLHIV
AQDDAYWWQARKEHERSARAGLIPSRALQERRILHDRTQKNGTDLDSKQNDDFDREQI
ATYEEVAKLYPRPGVFRPIVLIGAPGVGRNELRRRLIARDPEKFRSPVPYTTRPMRTG
EVAGREYIFVAREKMDADIEAGKFVEHGEYKGHLYGTSAESVKSIVNAGCVCVLSPHY
QAIKTLRTAQLKPFLIHVKPPELDILKATRTEARAKSTFDEANARSFTDEEFEDMIKS
AERIDFLYGHFFDVELVNGELVNAFEQLVQNVQRLENEPVWAPSMWVQ
Comments: CG18338 and CG13219 are two portions of a single gene, which encodes
a p55-like MAGUK subfamily member that we have named Skiff (Skf) (Genbank
AF495381).
We have sequenced three cDNAs (including GH12103) and found that they contain
sequences of both predicted genes. Examination of genomic splice
acceptor/donor sites is consistent with the gene merge. The predicted
full-length Skf protein joins L27 domains from CG18338 with the PDZ, GUK, and
SH3 domains from CG13219, and the resultant protein is more homologous to
zebrafish Humpback then either individual predicted protein. Northerns and
embryonic in situs using probes for either predicted gene show similar
patterns. Finally, mutations that affect CG18338 transcription also affect
CG13219.