Subject: LD11279 and LD20919
Dear Gillian,
following your suggestion a few weeks ago, I am including our
sequences
corresponding to cDNAs LD11279 and LD20919. I am sending them to you
as attachments in FASTA format. These sequences confirm
that this locus contains at least two different transcripts sharing
the
first exon. This common exon is non-coding and therefore the
transcripts code for completely unrelated proteins.
LD20919 confirms the predicted structure for gene CG8165. In contrast,
LD11279 is not completely identical to the predicted gene CG8176 as it
appears in Gadfly. When I blasted this sequence against the genomic
clones it revealed that the gene contains 12 exons instead of the
predicted 13.
Ibo Galindo
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Dr. Maximo Ibo Galindo
School of Biological Sciences
University of Sussex
Falmer
Brighton, East Sussex BN1 9QG
UK
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>LD11279
GAAAAATCGAATTTTTCGCTTCAAATCGACTGAAGTTTACACGGGCTAAAGTATTACAGAAGTTTGGCTCAAGCCATTAC
ACAACATGCAATAAGTAAATTATTTACATAGACAACACTAAGCCTAAGCAAGATACTCTCCAGCAACAAATGCCCATGCA
TGCTGAGGACGGAGCTGAATCTGATGATGAGGAGTCCAGCCCAGGCAGACATTAGCATCGTAAGGATCATACTACTAATT
TGGCGCCCATATAGAAGTCAAGTCAATTACAACGCCAGAAAGCCTTTTATGTTAATCGAGAGTGGAAAGGGAAAATAATT
TTTTTGCTAAACTAGCCTAGAGATCGAAATATTAAGAATACAACTAAAATGACGGTGGATTTCAACGATTATTTTTGGGG
GGAGAAGAACAATGGCTACGATGTTCTGTACCACAACATGAAGTTCGGCCTGATTGCCAGCAAGGAGTTATCCGAGTTTC
TGCGCGAGAAGTCTAACATTGAGGAGCAGAACTCAAAGATGATGTCCAAGCTGGCCCACAAGGCTGGTACACTAAATAGC
ACATTCGCTCCTGTTTGGACCATACTGCGAACATCGGCAGAGAAGCTCTCCACGCTCCACCTGCAGATGGTGCAAAAGCT
GACTGAGCTGGTCAAGGATGTTGCCAAGTATGCCGACGAGCTGCACAAAAAGCACAAATCCGTCAAGGAGGAGGAATCGC
AGACTCTTGAGTGTGTCCAAGCCATCCAGACGTCGACGGTGGCAGTGCAAAAGTTGCGCGATCTGTACGCCAGCAAGGTC
CAGGAGCTGGAGAAGCTGCGCAAGGACAACGGCTCGCACAAGGATGCCGAGAAGCTAGAGAGCAAGCTGAAAAAACTGCA
GGAGGAGTACAAGGCTTTGTTGGACAAGCATAACCCCATTAAGAACGAATTTGAGAGACGCATGACACAGACCTGCAAGC
GCTTTCAAGAGATTGAGGAGGTGCACCTTCGTCAAATGAAGGAGTTTCTGTCCACGTATCTAGAAATGCTGCAAAACAAT
CACGATATGGTGGGTCAAGTACATTCCGACTTTAAGAGGCAGTTTGTCGACATGTCTGTTGATAAACTCCTGGAACAGTT
TGTGCTCAATAAATACACGGGATTGGAGAAGCCAGAAATGCCTGAACTGGAGTATGTGGCCTTGTCGAGTGGTTCTCGCC
TTCAACAGCCGCAGGTCTCGGCCAGCGCATCTAACTCCAATTCGGCCACCAGCATTCAGGCTGCTCTGCGGGCTGGAAAT
GCAGCAACGACGGCCTCGGGCAGTGCGGTGTCCATGGATCAACGGGGCGATGCCTTGGCGGCAATCTCTCTGGGCAGCGG
AAGTGCAGCCAGCGGTAGTATACCCTTTGCCGAGGATCCGATACTGGGAGCTATGGGCTCTCCGCGTCCCACATCGCCGG
ACATGCTGGCGGGAGCCAATGCATCGGGAATACAATCGCAACCGACTGGTGGAGCCACATCCACGGTGAAAAGCCTGCGA
TCCTGGTTTTTGCCCACTGCCAAACAGCAACCGTCTGCAGGCGCCGGAAATTTCGGTATGGCCGGAAGTGGCAGCACCAA
CAATACCACCACCACGACAACAGCAAGCAACAATAGCAACAACTTGAACACCAACAACACAACAGCAACAACTACAACAA
CAACTGGCACGAATAATCTTACCACAAATATCACCAAAAATTCCCCTAATTCTGAGGATACTAGCAATCAAGTCTCTGGC
ATTTTACGCAGTCGTCGCGACAAGACAAAAACCAAAAAGGCTAAGAAAAAGAAAGACAATGAGGCCGCCGAGAACATACA
GGAAGAACGCCTCACCAGTTTGGATCGAGGCAACAACACGCTGCTGGACTATGACGACCATGGGCGCAGCATGAAGACGC
AGAATTCCAGCGGCAGTAGCGCTGGCGGTGGCTTGGCCGCCCTATCGGTGACCTCAGCTCAAGGAAGCGTTGATTCTGCA
GGAGCAAGTAGCATGGGCGGAAGCGGAGGTGCGGCAGTGGCCAGTGGGAGCCTACCCAATGCAGGCGCCCTGACCAACTC
GGCTTCGATCACAGCTCCGGGCGCCGCAAGTGGGACCGGAACTGGATATGAGGTCGATGAGGAGGGATTTAGCATACAGC
CTGCTAAGGAAATAGCGTGGGAGGAGGGCCAGGATAAATCTGGCAGCTTCTATTCCGACTCGGATACGGATTCGGACAAT
GACAAACAGGAACGTCGGATCCATGTCAGCATTAAGCCTTTGAAGAATGGTCAAGCGCCCATGTCGGCCAGCGTGGATGA
GCTGCGTGCCACCGTGGAGAACATTTCGCTTTCCCCAACTGGAGTTTTTTCACATCACAGCCAGCAGTTGCAAGCAGCGA
GAGGAGCTCCGGCGTCGCGTCAACAGTCGCCGGAAATATCGAATGCCTCCACTCCCACGGCCAGTGTGCATCCTTATGCC
CCGCTGCAGAGTCCCACGCTGTCCAACAATAATAACGCAAACAACACGACCAACAGTCGTTATGCCGATCTGGGCGATAT
CTTCTCAGAGTTGGGTGACATGAGTATGTCGGCGCCCTCATCGGCGACGCTGGGAAAACCTCCCGGAAGGCAAATACCAA
CACCGACGTCAGCGTCAACAGGTGGAAGCATAGCCATACCACGTCCGCCATCACGACGCTCTGATATGATTGCCATTGGA
CCGGCGGCAACAGGGACAGCGCCAGGTCGCGGCAGAATGTCCCCGGCACCGGTGACGGCCATGAATCGAGCCGAGAGCAT
TGGCAGTCTGGAGTTCCGTACGGCTATCGGGGGAGTGGGATCCTCGCGAGGCCCCTCTCCACTTACAATCGGTATTTCGG
ACACTATACCGCTGGCTGTGGCATTCCACGAAATCATACACGCATATTTTCGGGGCAGCGATGAGTCGCGCTGCCAGGTG
AAAGTGTCCGGTGATATGATGCTATCATTCCCAGCTGGCATTGCCGGCCTTTTAGCCAATAATCCAAACCCAGCCAAGCT
GGGTTTTCGCATTAAGCACGTTCAGAATCTGGAAAACCTGGTGCCTAACGGAAAACTGGTGCAAATAGATCGCCAGCAGT
CGAGTTCTGTGAGCACGATGCTGGAGTTCAATATGGGTGCTTTAACGGCCCTGCTCCGAAGGCAGGCGGAGAATAACCCG
ACTGCCTCCTACTTCAACGTGGACATACTCAAGTATCAGGTGCGCACGAAGCCGGGAGCCAGCTCGTGTCCCTTCCAACT
TGTCAGCTACTGGAAGTGTGAGTCCAGCTACACGGCCCTTAAAGTGGACTACAAGTACAATAACCACGCCATGGCCAGTG
CCTCGCCCCTTCTGAATGTCACGCTCTCGGTGCCCGTCAATGGATCTGTGCGCAATGTCCAGTCAAAGCCGCATTCTGCC
TGGTTGGGCGAATCGAATCGTTTGGTGTGGAACTTTACGGACATCTCGCAGAATTCACAAGACGGCGGAGTGGGGACGCT
GCGGGCACGCCTGGAGCTTAACGATGGTCCCTCTACACCAGCACTACTGGCCACTCAGTTCAACTGCGAGGGCACCACAC
TTTCGGGCATCGAGTTTGAGCTCCAGGGCAGCGGCTATCGCTTGTCGCTGGTCAAACGGCGCTTCGTTTCTGGAAAATAT
GTGTGCGAAGGCGATGGCATTCGCAGTGGAGCCACACCCACGCCTCCGTCGGTCGGGTCCACCAGTCCGTACTCGGCGAA
GTCAAATTAGTTACTCCCCACTCAATAGACGGCAAATGGGAACGAAAAATGGCAATATGGCAATAGGCTCGTGAAAAACT
AGAGAAATTTTTGTAACTAATCTTAAATATGAGACAACCACATATACACCCAAACCATATTGAATTAGGCACACGAATAG
AGGAGCCTACATAGTTTTCTCTGAGCTTTTTATTATACCCCCCTTTCTTGATTTCGAAATGGACATCACAATGCGGAATG
AAATTGACAAACTTTAAGATTTCCACAGATCGAAGCACGACAAGATTGAGGAAACTGGAAATTCTATCAATTCGCATTCC
AACAGTTTAGATCCAAACGAAATCGAAAGTGGGACCTATTATGTATTTTTGTACACACAAAATTCTGTTTTTCACGCGAA
ATGTTGAATCTGCCTGCGGGAGGTCCGAAATGCGGGCCTGCAAAATCTGGATGTATTATTTGTTATATATATACGTATGC
GAGTATATATTATTAAAGTGTATTTATTATGTTTAACTGAATATGATGTAAACGAACGATATTGTATGTAGCTATGTAGA
TATCTAAAAACAACACACCGAACGTTAATTTTATGTTCACTTTTGTACACACAACCAAATCCAAATGTATTAAATGTAAG
ATACAAAAAAAAAAAAAAAAAA
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>LD20919
GAATTTTTCGCTTCAAATCGACTGAAGTTTACACGGGCTAAAGTATTACAGAAGTTTGGCTCAAGCCATTACACAACATG
CAATAAGTAAATACAGAGGAGCTAAAATGTCGCAAAAAGAATTGGCGGCTCTGGTTGCCACGAGAACGAGTCGCAAACGC
ACCATATCCTCGACCTCATTCTCTATTTCCACTCGCACTGATCCAAAATCCATCATAGAGATACACATATTCAATGACTC
CTTGCCCATAAAAAGAGCTTGCTGCGAAGTGGAGAATCCAACTTTAAAAGCGGAACAGCGAGTGCAGCAGGAAGAAGAGA
GCCTGGGCCAAGTGCCGCCGCTGACCGAGGAAGAGCAGCAGCGGCACGATGAGTTCCGAAATAGTGGTTCGATATTCCTG
CAGGATTTGCCCTGCTTTAAGCTGCAGCAGGCACAGCCGATAGCAGGATCATCACCCATTCCCAAGTGTCGGGAGTGCAG
AAGAAGAAATTTGGCTACAACAGAGGGCGAACCCAGTTCAGTTAGCGACGTTTACTGCCGGTTCTACGAgTTCCGACGCC
TTCAGTTCAATGAGAATGGAGAGCTGTGCGTGGTTGGCTTCCCAAATCCCTACAGCGAGCCCTCACCAGAGGACATCGCC
ATTTGGCAGCCGGATAAAAATACACCGCCGACCAGCGGTTACATGGATATACAGGTTTGCAGGTACATCCTACTCCATGC
CGGCGACCAGTTCTGCTATATTTGGCACCAGGAAGCAGAGGCTTTAAGTTTGCATCAGAATACGGACGGAACCATTGCCT
GGAAGAAGGCCGTAAAAGGAACACGAGAGATTTGCGACGTGTGTGACACCACATTGTTCAACTACCATTGGACTTGCCGC
AAGTGCGGCTTTGGAGTGTGTCTGGACTGCGTTAAAGATCGCAAGGAGGGATTGCGACTGCGGCGAGCGGAGAACGCTGC
CCAAAAGGGATGCGATGAGTACCACTGGCTGCTCTGCAGCGACCCCAGTGGCCCACAGGAGCACGTCCTTACCGAGCTGA
TGCTAACACAGATCATAGCCGGAGATGCCCTCAACGTCTTGGGCAGGCTGCTGCACGAGGTTCGTACTTTGTGGCAGGTG
CCACAGGTCTGCGGCTGCCTTCTGAGCAAGCAAGCGATTGAAGATGCACAATCAAAGGAAGTGATCCAGGATATGATTAA
GGAGTCTCAGCTGAAGCAGCACACCAGCTACTCGTCGCTGGCCTCCGAGCAGAAGGTGCACCAGCAACAGCGCCTCGATC
AACTGCACGCCACGAAGTTGGAGTTTGCCAGAGAGCTAGGTGTAGATTATGTTCCAGGTCGAGTGTGGACCAAGGAAACC
TTGGGAAAGGATCCAATAACAACGGCGTTTGATAATTTGAAGCACATTAATTTTCTGAGAAAGGGATTGGCCGGCTTGAG
AAGGTTTCTTCCACCAAGGACCATGACTTTTGCTTACTCCACTCAGTTGGCTCCAGGAGTTCCTCACGAGTTTCTGTGCG
ATGGCAGACTGCTTAGACTCACCGATGCCATGCATCCGGATAATCGAGTTCTTTTCCAGGAGGTTTGGAAATGCGGCCAG
CCAGTTATGATTTCGGAGGTGGCTCGCTCTTTGAACTTAGACTTATGGCATCCACAAGCCTTTTGCCGGGACTTTGGCGA
CAAGCCCAATGATCTTATTAATTGCCTGAATGGCAATCTGGTGCCCAATCAGCCAATGCGACACTTTTGGGAGGGTTTTC
AGTGCATGACCAAGCGTCTGCCAGATGCATATGGCAAGCCAATGCTCTTAAAGCTTAAGGATTGGCCGCCGGGTGATGAT
TTTGCCGAAATACTGCCCACCAGGTTTGCTGATTTGATGAAGGGACTGCCCATGCCGGAGTACACACTTCGCACGGGAAA
CCTTAACATAGCTAGCTGCTTACCCAAGATGTTTGTGCCACCAGACTTAGGACCCAAAATGTACAACGCATATGGCTCAG
CATTGCACCCCGACAAGGGTACAACCAATCTTCACTTGGACATTTCGGATGCAGTGAACATAATGGTTTATGTGGGAATA
CCCCAGGATGGGGACACGAGACCTCAGATGGCGGCCACGCAGAAAGCCATAGAAATTGGCGGATGCGATTACATCACTAG
AGCTCGTTGCCAATCGCCAGATGTATTACCCGGAGCACTTTGGCACATCTTTCCAGCTCGCGATGCAGACAAAATAAGAG
ATTTACTTAACCGCGTGACCCTGGAGAAGGGTTTCCGTCTGGAGCCGGATCACGATCCAATTCACGATCAGAATTGGTAC
TTGGACGACAAGTTGCGAGCTCGTTTATTCAAAGAGTATGGCGTGGAGGGGCATCCGATTGTCCAGTGCTTGGGCGACGC
AGTCTTTATACCAGCTGGCGCCCCACATCAGGTTCAAAATCTCCACAACTGTATTAAGGTGGCCGAGGATTTTGTATCGC
CGGAAAATATCACGCACTGCTATCATCTAACTCACGAGTTTAGAAGGCTTTCGCACTCGCACACAAATCACGAGGACAAG
CTTCAGATCAAGAATATTATCTACCATGCCATCAAGGACTGTTGCACCATCCTGACCAGAGCTGTGGATGAGAGACTTAA
TGCGGAATTAACCAAGCTAAATGCAGATTAGAATTTTCTTACCTTTTAGTTTTTTTCAACTATTTAACATTTATTATTAT
TTTTTAAGATCAAAAATTGTCAAATATTCAATAAATAACTTACTTAGAAACTAAAAAAAAAAAAAAAAAAAAAAAAAAA
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