Subject: FlyBase query: snoRNAs
Dear Dr. Steitz,
I have just curated your paper for FlyBase:
Tycowski and Steitz, 2001, Europ. J. Cell Biol. 80(2): 119--125
which discusses the genes 'dUHG1' and 'dUHG2' and the snoRNA genes in
their introns.
It would be really useful to FlyBase if we knew the sequences of dUHG1,
dUHG2 and the snoRNAs so that they could be annotated correctly onto
the genome. Do you have these sequences (preferably in FASTA format) ?
If you send us the sequences, we would record your e-mail as a personal
communication to FlyBase so that users could see where we got the
information from, and we would use the sequences to annotate the genes
correctly,
Gillian
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Gillian Millburn.
FlyBase (Cambridge),
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Subject: Drosophila UHG genes
Dear Dr. Millburn,
In response to your email to Dr. Steitz, I am sending you sequences of
Drosophila UHG1 and UHG2 cDNAs as well as snoRNAs encoded within the UHG
introns. Note that some of the snoRNA genes are found in several introns
(Fig. 2 in our paper); the variants of the same snoRNA differ by only
few nucleotides. I am sending you only one representative sequence of
each snoRNA.
The 5' ends of the cDNAs have been experimentally mapped and the 3'
ends terminate at the first AAUAAA polyadenylation signal; the authentic
3' ends are not known.
Please let me know if you have any further questions.
Sincerely,
Kazimierz Tycowski
Research Scientist in Joan A. Steitz Lab
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>U14snoRNA.Seq
ttttactgatgattaacttcaacaccttttgcggtttccaccagaaagcttcggcttaatgatggtctaaggcgtctga
ct
>U15snoRNA.Seq
ctcaaatgatgaaacagatgacgagactgaaggaacaaattctcgacttggcgctgtatttttcagtgcctcgttatat
atttgttcatttaagatgattcggcgatctgatg
>U27snoRNA.Seq
gttctgtgatgtcaaaccaatagacaagcatataaccgaacaatcatgttgattttcacacgactgagc
>U25snoRNA.Seq
cttctctgatgatcttttacacagacctgtactgagctcataaccgtgcagaaaattgtaaactgaaa
>U29snoRNA.Seq
actcaatgatgaaacatttacccagctcgctttgatattcaatgaagagaactgttccatatccaacgcaaaaataaac
tgctgact
>U31snoRNA.Seq
cttacatgatgattttattcgtaccgccccagtctgaagagacgtgttgattggttatttatactgata
>U76snoRNA.Seq
tgtaaatgatgatttattattatttgctactcttgaaggtcattgatgaatactttcaccttaaaacctgatg
>Snr38snoRNA.Seq
gggcaatgatgaacactttatttaagccaaacagttatccctgtcataagaataattgatgataaaagttgctgatt
>Dros-UHG1-cDNA.Seq
cacttctttttttcgttcaaaatggacggtcgcgttttctgcttgaattctcaactaaaatttaacaaatgggatgttg
tgcggtagcaggaaatggaactgaaaataactatggaaaaaacagtttttatggctttctgtcaaatttatccacacgt
gtctcaaagtaagatggtctattcgaataaaagagatttcgcttacggcaaagatagacggaagattatatgattcgaa
ataagttcttataaattcctagggatctgtaatgtcggactcctattttccttaattccggaaaaatcaaaatgtttag
aatttaagtcagtgatcctggccgtctgcttaaggaaaaatgagaaaatgagaaaatggttgacacaaatcataatcat
aatcaaacaaaatatacaatccacggataaatcttttctcagaacaatggaacacagaaatgcggtcaagtttggaaat
atgagaaatattttttcaagtttactcgttgttttcactgggttttgcgctttgtagttaaacgggaacgcaggaaagc
ttagatattgaacgaataaaatttaaaaatggtatttgcacactatataaatcaagctgcgaaggaattttggtgatta
atgaagattcgctaatatcaacatattacaaaataaaacccgattgaggatggctaaaaatctaaaggtattccccgtg
ttgactgacagccgatttgcaatggctttttagtacaaggtcttcataatatgcagtacccaagttggtttggattata
atatgtatttccaagatgtttgaagattaccagggaaaaagtctatctaccctgcaagattattaaggcttgaactaaa
gagcggtcgttttgcatggaagatcccaaatgaacgcagaatattaataaggtaatacctgacatctggctttgtgatt
cagctgaacttttgtggtatcgtgcgaattgcaaagatcgaccaacgccatttcactccaaaccccaaattgctgtagt
catttaaacaaataaa
>Dros-UHG2-cDNA.Seq
cattttatttcaagactcgaaatgtgaacaatattgaaaccctaagcatttggataaatcgaaatccgggcactccacg
tgcatggtgcatgtgagacacctcgggacaacatgtgagcccgttgagacaccttgggcactgagcccactggagacgc
cattgtcacattggagaatttacattctggtttgaggttatgtatgggtccagctgcagcagcgagcacatggatttac
cgataaagatcaactcagaaatgtggtttatcgcaggcaaatataactaaatgagctcgtttattcgactgtataagag
tgacgttgccttgtgtaaaagatcttgatataaaaagaattggacatcttcaaatactaaaataaa
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