Hi Gillian,
My name is Greg Rogers, I'm a postdoc with David Sharp at the Albert
Einstein College of Medicine. I used to work for Jon Scholey and he
forwarded your e-mail to me. I was surprised and impressed with you
question, you guys really keep up on this stuff which is
fantastic. Anyway, the real story and answer is that there are actually
two kinesin motor genes near this position one at KLP59C (CG3219) and one
at KLP59D (CG12192), they are approximately 17Kb away from each other. The
searching in FlyBase does recognize CG12192 as a kinesin motor at 59D1
position, so we're calling it KLP59D. The CT number for KLP59D is CT9848
but there are some mistakes I believe. Anyway, KLP59D is a real gene that
is transcribed, and have sequenced two identical full-length ESTs for
KLP59D (AT18571 and AT12557). Let me know if you want cDNA sequence for
KLP59C and KLP59D \- we have them for both genes.
Take care,
Greg
Dear Dr. Scholey,
>
>I am curating your abstract for FlyBase:
>
>Rogers et al., 2000, Molec. Biol. Cell 11(Suppl.): 569a
>
>Three microtubule-motors, cytoplasmic dynein, KLP59D and KLP38B,
>function cooperatively to position chromosomes relative to spindle
>poles in Drosophila early embryos.
>
>You mention 'KLP59D' in this abstract. We don't have a record of this
>gene in FlyBase, but we do have a record of a 'Klp59C' (which
>corresponds to the gene prediction CG3219). Is your 'KLP59D' the same
>gene as 'Klp59C' ? If it is not the same gene, do you know which of
>the Genome Project CG annotations your gene corresponds to, so that I
>can rename the CG in FlyBase,
>
>thanks,
>
>Gillian
Subject: Re: KLP59D
Hey there,
Making the e-mail a personal communication would be just fine. I've
included the KLP59C and 59D cDNA sequences attached as MSWord format
files. If you have trouble opening them or anything, please let me know.
Greg
..
KLP59C cDNA Sequence (also know as CG3219)
ATGGATAAGTTGTCGATCGAACAGAAGATTTTCATCCGTCGCTCGGACGGTAGAGTCCACTTGGCGGAGATTATCAAAC
TGGAGGGCGGTGACTCCAAGCTGATTACGGTGGAGTGGCCCGAAGGACATACGGTGCGTGGTAAGGAACTGCCCCTGGA
GCTGGTGGTCCTGATGAATCCTCATATCTTCGACTCGCCCCGCTGCAGTGGCGGCAATGCGGCGTCAGCCAATCAAACT
GCCTCCATATCGCCACGTTCAATGAAACAACGAATAGCGACTGGGAGCTTATCTCCTGTACTGGCCACTGCCCCGCCAC
GCCAGCAGACGGCGCCGCCAGTTCGAGAGGACGAGGTGGTGCACCAGGCTGAAAGAATGCGAAAAGAACGGGAACGGAG
GCGAGAAGCACAAGCCAGGACTCGTCTAGATCGGGAGCAGGGGAAGAACGAAGATCCGGGAAATCCCAACTGGGAAGTA
GCCAGAATGATACGACTGCAACGCGAGCAAATGGAGAGTCAGCGGGTGAGAAGTGGTACTACGAACGAACGAATCAATT
GCCACCAAATTATGGTTTGTGTGAGGAAGAGACCACTGAGGCGCAAGGAGCTGGCTGACCGGGAACAGGATGTGGTCAG
CATTCCGTCTAAGCACACATTGGTGGTCCACGAGCCCCGCAAGCATGTGAACCTGGTCAAGTTCCTGGAAAATCATAGC
TTCCGTTTCGATTACGTCTTCGACGAGGAGTGCTCCAATGCCACGGTCTACGAATTCACAGCCCGACCCTTGATAAAGC
ACATTTTTGATGGCGGAATGGCCACGTGTTTCGCCTACGGACAAACTGGAAGCGGCAAGACCTATACGATGGGTGGTCA
GTTCCCCGGAAGGCATCAGAGCTCAATGGATGGCATCTATGCAATGGCCGCTAAGGACGTGTTCTCCACTCTAAAGACG
GTTCCCTATAACAAGCTTAATCTGAAAGTTTACTGCAGCTTCTTCGAGATCTACGGCACCCGGGTGTTCGATCTCCTGA
TGCCTGGCAAGCCACAACTGCGTGTCTTGGAGGATAGAAACCAGCAGGTGCAAGTGGTGGGCCTCACCCAGAATCCAGT
ACAGAACACCGCCGAAGTTCTGGACCTACTCGAGTTGGGCAATAGTGTCCGAACCTCGGGTCACACCTCTGCCAATTCC
AAGTCCTCCCGATCGCATGCTGTGTTCCAAATCGTGCTGAGATCCGCGGCGGGCGAGAAGCTACACGGGAAATTCTCGC
TTATAGATCTGGCCGGGAATGAAAGAGGAGCGGACAACAGCTCGGCGGATCGACAGACGCGCCTGGAAGGATCCGAGAT
CAATAAATCGCTGCTGGTCCTCAAGGAATGCATTCGCGCTCTGGGGCGCCAGTCGAGTCATTTGCCATTCCGTGGCTCC
AAGCTGACCCAAGTCCTGCGGGACTCCTTCATCGGAGGTAAGAAGGTGAAAACCTGCATGATAGCCATGATCTCGCCAT
GCTTGCATTCGGTGGAGCATACCTTGAACACGCTGCGTTATGCGGATCGGGTGAAGGAACTAAGTGTGGAGTCGATCCC
ATCTAAACGGATGCCCGATGCCAACCTTGGAAGCACTTCCATGTCAGATATTGTTTGCCAGTCGTCCACCCAGCGACTC
TTTCCATGTGCCTCCTCCACATCGATGCCAGGTGGTGGCAACCAAGCGCAGCAACACACCAACACCGCGAACGACTTGA
ACAGATCCCAGAAACCAACATCTAAGCCCACTTATCCCACATCCGGTCAGCAGCTGGTCCAGAGGAAGGGAAGCTCCCA
ACGGGAGGCCTCCATGATGCTGACCAAATCCCTGGCACAATTCCGAGGGCGCAACTTTCCCTAA
KLP59D cDNA Sequence (also know as CG12192)
ATGGATCGCATCAAAATTGGCGAGCAGCTCCTCTTCCAGAGGAGTGACGGGCGTGTCCAT
CAGGTGGTCTGCACTGCCAAGAACCTGGAACGGGATTCCATCACGGGCGAATGGACCGAG
GGCACGGTGGTGAAGGGTAAGGAAGTGCCATTGAGCACGCTGATTGGCATCAACCATCAT
ATATTCGCGGAGAATCAACCGCCGATATCGAAGCCACCGAGCTCCTTGCTGCCGAAGCCT
CGAGGCTCGAGTCCGACCGGAGGACCTAAGACCCACACTGGAAATCCGTATGCAGAGCGC
ATGAGAGTACAGGACGGTCGCAGTAAAATTGCCACCAGAAAGACGGATCCTGCAGCGGTC
GGAGCGGGAAATCATGAGCTGGGTAGAGCGCACACCCCTCGTGTGCCAGCCGGTAGGCCG
GACCGGGATGCCAGCCCCACCCAAGGTCGCAAGTCACAGGGTGGCAACAATAGCAACCAA
CGCTTTTCCAGTGTAGTGAGGGAGGTGAATCGCATGAAGGAGCAGAGGGAGAAGCGAAGG
GCTCGCCAGGCGGAACAGCTCCAGGAGAAGGATGCACTGCGTCGCAATAATCCGGGGAAT
CCCAACTGGGAGGTGCACTGTTCAGCAAATTACGGTGTGTGTGCGAAAACGACCCATGAG
TCGCAAGGAGGAGAACTCCAAGAACCTGGACATCATCACAGTTCCCAGTGCCGACAGCCT
GATCGTCCATGAGTTGCGCCTCAAGGTGGATCTCACCAAGTTCCTGGAGCACCACAAATT
CCGTTTCGACTACACGTTCGACGAGGAGTGCTCCAATGCGCTGGTCTACGATCACACTGC
TCGTCCGTTGATCAGAACCATGTTCGAGGGCGGCAATGCCACTTGTTTCGCTTACGGACA
AACTGGCAGCGGAAAAACGCACACCATGGGCGGAGAATTCTTCGGAAAGGTTCAGGATTG
CGGTACCGGGATCTACGCCATGGCAGCTCGCGATGTCTTCGAGGAGGTATCGCGCCCGGA
GTACCGGCAAATGGGTGCCAAGATTACGTGCAGCTTCTTCGAAATCTATGGCACCAAGGT
GTTCGATCTCTTGCTACCCAACAAGCCCATGCTGCGGGTCCTAGAGGATGCCAGGCAGCA
GGTCGTGGTGGTGGGCCTAACGGAGATGCCGGTGACCAAAGTGGAGGATGTCCTGAGACT
GATTGAGCACGGCAGCAAAGAGCGCACTTCCGGCCAAACATCGGCGAACGCCAAGTCATC
GCGTTCCCACGCCGTCTTTCAAATAGCACTCCACTTTCCCGATTCCTGGGGCCCACACGG
CAAGTGCTCCTTTGTGGACTTGGCGGGCAATGAACGCGGGGCGGATACGCAATCCGCCGA
TCGTCAAACTCGCATCGAGGGAGCCGAGATCAATAAATCTCTGCTGGCCCTCAAGGAGTG
CATTCGAGCCCTCAGCCGGCAGTCGAGTCACCTTCCCTTCCGTGGCTCCAAGTTGACCCA
AGTGCTGCGCGACTCCTTTGTCGGCGGCAAGAAGAACAAGACCTGCATGATTGCCATGAT
ATCGCCATCCATGAGCTGCGTGGAGAATACGCTCAACACTCTACGTTACGCAGACAGGGT
TAAGGAGCTCATAGCCAAGGAAGACGAACACTTGCAGTCCGTGGAAGGGGATGGAGAGAA
GTCTCCCGATCTCAACGAGGAATCAGAGCCAGAAATGATGGCCGACGAGGAGGGCGATGA
GGAGCCGGAGGATGAGGACAATCAGCATCTAACCATATCCTCGGAGGAGGCAAGCTCCTA
CAACAACATGAGTTATGACATGAGCTTTAACCACACCCTCAACATTCTGGGTCCCTCAAG
GAACGTGGATATCCAAGAGGTGGCCGAGCAGCACGCACTTTTGGTAGAGAATCTAGAGAC
TTACGCTCACAATTTCCGGCAACTGAAAACGGATAAGGAAATAGAGCAGTACACGCAAAA
TTCAGAAAGCGCATTGATGAAACTGCTAGCAATGGTGAATAGAACGCGGGATGTAACGCA
CAATTACAACACTCAGAAATTATTGAAGGAAGAAAACCAAAATGCATATAAGAAGGGCGA
GCTGGATGAATCCGAGTAG