Subject: Andante Hi Rachel, I wanted to add something to the discussion of Andante and Adrocam (i.e., your previous emails with Paige Pavlik). It turns out we have finally resolved a discrepany concerning dusky (dy) and Andante (And), which we had believed for a number of years might be the same gene. We now know that they are two different genes within region 10E2-3. We recently published some old work on dusky in MGG and are now writing up the work on Andante. The 'long and short of it' is that the Andante mutant carries two independent EMS-induced mutations, one in dy and one in CKII beta. It is the one in CKII beta that is responsible for the circadian long-period phenotype. Dusky mutations have no effect on rhythmicity. Thus, the name of Andante ought to be CKIIbetaAnd. I hope this helps. Regards, Rob \-- F. Rob Jackson, Ph.D. Department of Neuroscience Tufts University School of Medicine Boston MA 02111 USA Subject: Re: Andante Dear Rob, All very interesting \- nice that it is becoming clear. So what I need to do is extract all reference to Andante from the dusky gene record, and move it into that for FBgn0000259. I'd appreciate a bit of help with some aspects of this \- so please excuse a few questions.... Firstly, I do not believe that any of the molecular information in the gene record for dusky applies to Andante, does it .... by 'molecular information' I mean these data-bits: \*g M84606; AAA28490 \*m SPTREMBL:Q24328 \*m SPTREMBL:Q9VYU7 Is that correct? It seems a very close call as to name precedence, but you seem clear that CkIIbeta takes priority so I'm happy to go with that. Alleles that we currently refer to as dyn1 (FBal0031147), dyn3 (FBal0031149), dyn4 (FBal0031150) and dy73 (FBal0003275) all have 'locomotor rhythm defective' and all but dy73 have 'eclosion rhythm defective' as features of their phenotype. Are these all double mutants too? (They do not seem to have a common ancestor, at least not according to the info we have on them). They were all discussed in FBrf0054173 == Newby et al., 1991, Genetics 128: 571--582 and it seems a bit strange to be removing all Andante rhythm stuff from the dy gene record while leaving this in. Finally \- I will have to name the dy allele on the Andante mutant chromosome as something, even though it will have no rhythm phenotype associated with it. I would rather it wasn't dyAnd. Do you have a line designation, or an amino acid alteration (e.g. Y742N) that I could use for the dy allele (e.g. dyY742N or whatever). When we have ironed out all these details I'll curate your mail (the whole correspondence, probably) as a personal communication from you to FlyBase, to bring about the change in a trackable way. all the best, Rachel. Subject: Re: Andante Hi Rachel, I will try to answer each of your questions about dy and Andante in the appropriate section of your email below. If you still have questions, please contact me again. Best, Rob \-- F. Rob Jackson, Ph.D. Department of Neuroscience Tufts University School of Medicine Boston MA 02111 USA \------------------ >Dear Rob, > >All very interesting \- nice that it is becoming clear. So what I need >to do is extract all reference to Andante from the dusky gene record, >and move it into that for FBgn0000259. I'd appreciate a bit of help >with some aspects of this \- so please excuse a few questions.... > >Firstly, I do not believe that any of the molecular information in the >gene record for dusky applies to Andante, does it .... by 'molecular >information' I mean these data-bits: >*g M84606; AAA28490 >*m SPTREMBL:Q24328 >*m SPTREMBL:Q9VYU7 >Is that correct? All reference to Andante should be removed from the dy record. This includes a portion of the mosaic analysis data showing a requirement for the rhythm function in the brain. However, you might wish to include a note in the CKIIbeta record that the Andante strain carries two hits, one in CKIIbeta and one in dy. The most recent and appropriate reference for all molecular information about dy is DiBartolomeis et al. (2002) Molecular Genetics and Genomics 267: 564-576. The entire molecular characterization of dy is in that paper. >It seems a very close call as to name precedence, but you seem clear >that CkIIbeta takes priority so I'm happy to go with that. I think it makes sense to use CKIIbeta since that really is the molecular function affected in Andante. >Alleles that we currently refer to as dyn1 (FBal0031147), dyn3 >(FBal0031149), dyn4 (FBal0031150) and dy73 (FBal0003275) all have >'locomotor rhythm defective' and all but dy73 have 'eclosion rhythm >defective' as features of their phenotype. Are these all double >mutants too? (They do not seem to have a common ancestor, at least not >according to the info we have on them). They were all discussed in >FBrf0054173 == Newby et al., 1991, Genetics 128: 571--582 >and it seems a bit strange to be removing all Andante rhythm stuff from >the dy gene record while leaving this in. We believe that the dyn1, dyn3, and dyn4 alleles are not really independent alleles but rather re-isolates of Andante, based on the molecular characterization of polymorphisms in these strains and Andante. Although dy73 is reported as being rhythm defective, it is not because of a mutation in dy or CKIIbeta. As indicated in Newby et al (1991), the factor causing the dy73 rhythm defect does not map to the 10E region, but rather to some other part of the X. >Finally \- I will have to name the dy allele on the Andante mutant >chromosome as something, even though it will have no rhythm phenotype >associated with it. I would rather it wasn't dyAnd. Do you have a >line designation, or an amino acid alteration (e.g. Y742N) that I could >use for the dy allele (e.g. dyY742N or whatever). This is a good idea \- the nucleotide change is a G to A transversion (characteristic of EMS) that results in a change from a Gly at residue 189 to an amber stop codon. You might want to indicate that DiBartolomeis et al (2002) refer to this mutant as dyAnd.
