Dear Dr Corces,
I am currently curating your paper for FlyBase:
Labrador and Corces, Genes Dev. 2003 17(1), 43--48
I have a quick question I was hoping you could answer for me.
At the beginning of your materials and methods section,
you cite 3 Bloomingtons stocks. 1521, 1522 and 4775. The first two
include the transgene construct,
P{UAS-GFP.S65T}
4775 includes:
P{UAS-GFP.nls}
Which also has a nls signal.
You describe a couple of new insertions of a UAS-GFP construct. Can
you tell me which transgene construct you used to make them?
pUAST- Hsp70:GFP4.1.3 
pUAST- Hsp70:GFP  22.21
Best wishes,
Chihiro
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Dear Dr Yamada,
Dr Corces just gave me your e-mail message asking for the origin of
transgenes pUAST- Hsp70:GFP4.1.3  and pUAST- Hsp70:GFP22.21 . I just realized
that there are several typos in the paper. These lines are in fact
pUASp-P/ T:GFP  lines, not pUAST- Hsp70:GFP  lines. Looking at the material and
methods of the paper I also noticed that there is another mistake concerning
the line pUASp-P/ T:GFP1.5  line.
The pUASp-P/ T:GFP  lines were generated in our lab using a GFP sequence
cloned in the cloning site of the pUASp vector. The name of the three lines
is corrected bellow. They correspond to insertions in different chromosomes
of the same P element.
I apologize for the mistakes. Hopefully this e-mail will help you for the
curation of the manuscript.
pUAST- Hsp70:GFP1.5  it should say pUASp-P/ T:GFP1.5 
pUAST- Hsp70:GFP4.1.3  it should say pUASp-P/ T:GFP4.1.3 
pUAST- Hsp70:GFP22.21  it should say pUASp-P/ T:GFP22.21 
Mariano Labrador, Ph.D.
Department of Biology
The Johns Hopkins University
Baltimore, MD