The following information accompanied stocks donated to the Bloomington Stock Center by Francois Huet and Bill Gelbart, Harvard University (11/02).
The following hobo-mediated deletions were isolated in Huet et al., 2002 (PNAS 99: 9948-9954) (FBrf0151478). The first two sets were described in detail. The third set was not described explicitly, but came from the same screen as the first set.
1. Deletions isolated as w+ y- progeny following hobo mobilization from P{wHy}01D01. All retain a P{w+mC=5'wHy}. The numbers give the size of the deletion in base pairs. The Roman numerals refer to the deletion class in Figure 3B. GenBank accession numbers for the sequences of deletion endpoints are given in Huet et al.
Df(2R)01D01W-L133 46568 II
Df(2R)01D01W-L186 105231 III
Df(2R)01D01W-L197 204791 III
Df(2R)01D01W-L053 320378 IV
Df(2R)01D01W-L149 398322 IV
2. Deletions isolated as w- y+ progeny following hobo mobilization from P{wHy}01D09. All retain a P{y+t7.7=3'wHy}. The numbers give the size of the deletion in base pairs. The Roman numerals refer to the deletion class in Figure 3A. GenBank accession numbers for the sequences of deletion endpoints are given in Huet et al.
Df(2R)01D09Y-M088 78060 I
Df(2R)01D09Y-M125 101979 II
Df(2R)01D09Y-M186 200920 III
Df(2R)01D09Y-M092 329337 IV
Df(2R)01D09Y-M073 353318 IV
3. Deletions isolated as w- y+ progeny following hobo mobilization from P{wHy}01D01. All retain a P{y+t7.7=3'wHy}. The numbers give the size of the deletion in base pairs.
Df(2R)01D01Y-R11 50175
Df(2R)01D01Y-R09 103508
Df(2R)01D01Y-R24 240928
Df(2R)01D01Y-R23 361566
Df(2R)01D01Y-R21 492864