Subject: Helping FlyBase: ADRC-10986 Dear Miho, We are currently curating the abstracts for the upcoming 44th (Chicago) Annual Drosophila Research Conference, for FlyBase. I am writing in connection with your abstract: Shattered encodes Anaphase Promoting Complex-1 (APC-1) and regulates G1 arrest in developing eye. You indicate that you know which of the Genome Project CG annotations shtd corresponds to. If you could let me know then I will merge the gene records for the CG and shtd. All the CGs have corresponding gene records in FlyBase already and we don't like to keep duplicate records for what is actually the same gene unless we can't avoid it. Thank you very much for your help, with best wishes, Rachel. \---------------------------------------------------------------------- Rachel Drysdale, Ph.D. FlyBase (Cambridge), \---------------------------------------------------------------------- Subject: Re: Helping FlyBase: ADRC-10986 Dear Dr. Drysdale, I appreciate your effort to updating Fly base. We are working for APC-1, it is CG9198. However, I am pretty surprised. I checked AE003499 genomic sequence a week ago, I found two alternative transcripts reported to CG9198. They are CG9198-RA(log form) and CG9198-RB(short form). We have APC-1 mutant, it is rescued by entire CG9198-RA ( long form), and shtd1 and shtd3 mutants have each mutation on N-terminal that is covered only CG9198-RA. Unfortunately, when I made CG9198 transgenic fly, it was 2 years ago, I did not have information about CG9198-RB. I did not make transgenic fly for short form CG9198-RB neither performed phenotype rescue. Thank you Miho Tanaka-Matakatsu
