P{EPgy2} is similar to P{EPg}. P{EPg} was first described in a
publication by Mata et al. (2002) Cell 101: 511-522 (FBrf0128569) and
the FlyBase ID for P{EPg} is FBtp0012862. The major differences are
that P{EPgy2} contains an intronless yellow gene module and lacks the
plasmid rescue module of P{EPg}. I constructed the plasmid pP{EPgy2}
from two plasmid precursors, p1462 and yellow-BSX.
1) p1462 was obtained from Pernille Rorth (EMBL, Heidelberg). This
was an intermediate used in the construction of pP{EPg}. She
described p1462 to me as being the same as pP{EPg} except for the
absence of the plasmid rescue module.
2) The yellow-BSX plasmid was obtained from Tim Parnell in the
laboratory of Pamela Geyer (University of Iowa). It was described as
having the SalI fragment of yellow-intronless inserted into the SalI
site of a modified pBluescript vector, pBS-X. This vector has the
KpnI site of the polylinker converted into an XbaI site. This yellow
SalI fragment is the same as segment designated by FlyBase as
y+mDint25.2(S,S) (FlyBase ID FBms0003824).
I digested yellow-BSX DNA with a combination of NotI and PspOMI.
PspOMI cleaves the same recognition sequence as ApaI (GGGCCC), but
generates the same 5' overhang as NotI (GGCC). NotI cuts yellow-BSX
at a single site in the polylinker sequences closest 3' end of the
yellow gene and PspOMI cuts yellow-BSX at a single site in the
polylinker sequences closest to the 5' end of the yellow gene. I
gel-purified the 5.8 kb fragment containing the yellow gene.
I ligated the NotI - PspOMI fragment of yellow-BSX with DNA from
p1462 that had been cut by NotI. p1462 has a unique NotI site
located between mini-white and the GAGA/GAL4-UAS modules. The yellow
fragment can insert in either of two orientations into the NotI site
of p1462. I recovered transformants in both orientations and named
them pP{EPgy1} and pP{EPgy2}.
--
------------------------------------
Robert W. Levis, Ph.D.
Carnegie Institution of Washington
Department of Embryology
115 West University Parkway
Baltimore, MD 21210, U.S.A.
------------------------------------
Subject: Sequences P{EPgy2} and p1462
X-Spam-Status: No, hits=-0.3 required=3.0
tests=BALANCE_FOR_LONG_20K,IN_REP_TO,REFERENCES,
SIGNATURE_LONG_SPARSE,SMTPD_IN_RCVD,SPAM_PHRASE_00_01, SUPERLONG_LINE
version=2.43
X-Spam-Level:
Lynn,
I'm attaching files of p1462 (including the plasmid vector) and EPgy2
(P-element only). Both were saved as text-only Word files in FASTA
format.
...Bob
------------- End Forwarded Message -------------
--Array_of_Hedgehogs_970_000
Content-Description: p1462.txt
Content-MD5: rAG/qKcYXsvPGKwUKbEKXw==
>p1462 from 5'P, including plasmid vector
catgatgaaataacataaggtggtcccgtcgatagccgaagcttaccgaagtatacacttaaattcagtg
cacgtttgcttgttgagaggaaaggttgtgtgcggacgaatttttttttgaaaacattaacccttacgtg
gaataaaaaaaaatgaaatattgcaaattttgctgcaaagctgtgactggagtaaaattaattcacgtgc
cgaagtgtgctattaagagaaaattgtgggagcagagccttgggtgcagccttggtgaaaactcccaaat
ttgtgatacccactttaatgattcgcagtggaaggctgcacctgcaaaaggtcagacatttaaaaggagg
cgactcaacgcagatgccgtacctagtaaagtgatagagcctgaaccagaaaagataaaagaaggctata
ccagtgggagtacacaaacagagtaagtttgaatagtaaaaaaaatcatttatgtaaacaataacgtgac
tgtgcgttaggtcctgttcattgtttaatgaaaataagagcttgagggaaaaaattcgtactttggagta
cgaaatgcgtcgtttagagcagcagccgaattaattctagttccagtgaaatccaagcattttctaaatt
aaatgtattcttattattatagttgttatttttgatatatataaacaacactattatgcccaccattttt
ttgagatgcatctacacaaggaacaaacactggatgtcactttcagttcaaattgtaacgctaatcactc
cgaacaggtcacaaaaaattaccttaaaaagtcataatattaaattagaataaatatagctgtgagggaa
atatatacaaatatattggagcaaataaattgtacatacaaatatttattactaatttctattgagacga
aatgaaccactcggaaccatttgagcgaaccgaatcgcgcggaactaacgacagtcgctccaaggtcgtc
gaacaaaaggtgaatgtgttgcggagagcgggtgggagacagcgaaagagcaactacgaaacgtggtgtg
gtggaggtgaattatgaagagggcgcgcgatttgaaaagtatgtatataaaaaatatatcccggtgtttt
atgtagcgataaacgagtttttgatgtaaggtatgcaggtgtgtaagtcttttggttagaagacaaatcc
aaagtctacttgtggggatgttcgaaggggaaatacttgtattctataggtcatatcttgtttttattgg
cacaaatataattacattagctttttgagggggcaataaacagtaaacacgatggtaataatggtaaaa
aaaaaaacaagcagttatttcggatatatgtcggctactccttgcgtcgggcccgaagtcttagagccag
atatgcgagcacccggaagctcacgatgagaatggccagacccacgtagtccagcggcagatcggcggcg
gagaagttaagcgtctccaggatgaccttgcccgaactggggcacgtggtgttcgacgatgtgcagctaa
tttcgcccggctccacgtccgcccattggttaatcagcagaccctcgttggcgtaacggaaccatgagag
gtacgacaaccatttgaggtatactggcaccgagcccgagttcaagaagaagccgccaaagagcaggaat
ggtatgataaccggcggacccacagacagcgccatcgaggtcgaggagctggcgcaggatattagatatc
cgaaggacgttgacacattggccaccagagtgaccagcgccaggcagttgaagaagtgcagcactccggc
ccgcagtccgatcatcggataggcaatcgccgtgaagaccagtggcactgtgagaaaaagcggcaattcg
gcaatcgttttgcccagaaagtatgtgtcacagcgataaagtcgacttcgggcctccctcataaaaactg
gcagctctgaggtgaacacctaaatcgaatcgattcattagaaagttagtaaattattgaaatgcaaatg
tattctaaacatgacttacatttatcgtggcaaagacgttttgaaaggtcatgttggtcaggaagaggaa
gatggctccgttgatattcatcacacccacttgcgtgagttgttggcccaaaaagatgaggccaatcaag
atggcaaccatctgcaaattaaaatgttactcgcatctcattaatattcgcgagttaaatgaaatttatt
tatcttctgcaaaactataaactatacatctcattgaaaaaaactaagaagggtgtggaatcaggcaatt
ctatctaaaatctagcgaatttgtttccaagaattgtaagcgttatatcatttgtttccactggaaccac
tcaccgttgtctgaataagtcgcacttttacgaggagtggttccttgagcaccgacagccaggatcgcca
caggaccgcccggaactgcatgaaccaggtggccttgtaggtgtacccattctccggctgctccagtggc
ttctccagatttttggtggccaacaactgctccatatcccgggctactttgctaatggcaaaattgtcgc
atatcttggcgatccgatcacgggactcgatctcccgtccgggcacaacggccaacacctgtacgtaaaa
gtccgccggattgtagttggtaggacactgggcacccacgctggataggagttgagatgttatgtaatac
tagatacccttaataaacacatcgaactcactaggaaaagaagtcgacggcttcgctgggagtgcccaag
aaagctaccctgccctcggccatcagaaggatcttgtcaaagagctcaaacagctcggaagacggctgat
gaatggtcaggatgacggtcttgcccttctgcgacagcttcttcagcacctggacgacgctgtgggcggt
aaaggagtccagtccggaggtgggctcatcgcagatcagaagcggcggatcggttagagcctcggaggcg
aatgccagacgcttcctttctccgccggacagacctttcaccctgccgggcacaccgatgatcgtgtgct
gacatttgctgagcgaaagctcctggatcacctgatccacgcgggccactcgctgccgataggtcagatg
tcgtggcatccgcaccatggcttggaaaatcaggtgttccctggccgttagggagccgataaagaggtca
tcctgctggacataggcgcacctggcctgcatctccttggcgtccacaggttggccattgagcagtcgca
tcccggatggcgatacttggatgccctgcggcgatcgaaaggcaagggcattcagcagggtcgtctttcc
ggcaccggaactgcccatcacggccaaaagttcgcccggataggccacgccgcaaactgagtttcaaatt
ggtaattggaccctttattaagatttcacacagatcagccgactgcgaatagaaactcaccgttcttgag
caaatgtttcctgggcgccggtatgtgtcgctcgttgcagaatagtccgcgtgtccggttgaccagctg
ccgccatccggagcccggctgattgaccgccccaaagatgtccatattgtgccaggcataggtgaggttc
tcggctagttggccgctccctgaaccggagtcctccggcggactgggtggccggagcgtgccgtagtttt
tggcctgcccgaagccctggttaatgcagctctgcgaagccgctccgctgtcaccctgcaatgatagggg
atctcaaatatcaactacaagcgttatgctcatctaaccccgaacaaaaagtaccccgaagtatcctacg
aagtaggtttatacttttatttattttttgtgcatctaggatcagcttaaaatatctggttgttatattt
tttgtaaaaaagaatatagtcgaaaatgaatgcctttagatgtcttgatcatgatatgatctcaaaaatt
gtcttatatagcgagaacagctaccagaataatctgtttcgtgtcactatttgtttgtgcaattgcggtt
tgggatttttgtgggtcgcagttctcacgccgcagacaatttgatgttgcaatcgcagttcctatagatc
aagtgaacttaagatgtatgcacatgtactactcacattgttcagatgctcggcagatgggtgtttgctg
cctccgcgaattaatagctcctgatcctcttggcccattgccgggatttttcacactttcccctgcttac
ccacccaaaaccaatcaccaccccaatcactcaaaaaacaaacaaaaataagaagcgagaggagttttgg
cacagcactttgtgtttaattgatggcgtaaaccgcttggagcttcgtcacgaaaccgctgacaaaatgc
aactgaaggcggacattgacgctacgtaacgctacaaacggtggcgaaagagatagcggacgcagcggcg
aaagagacggcgatatttctgtggacagagaaggaggcaaacagcgctgactttgagtggaatgtcattt
tgagtgagaggtaatcgaaagaacctggtacatcaaatacccttggatcgaagtaaatttaaaactgatc
agataagttcaatgatatccagtgcagtaaaaaaaaaaaatgttttttttatctactttccgcaaaaatg
ggttttattaacttacatacatactagaattcggtacccgcccggggatcagatccgcggccgctctagc
cccccctcgaatgttctctctcttctcttctctctctctttctcgaatgttctctctcttctcttctctc
tctctttctcgaggtcatcaagcttaggcctccaaggcggagtactgtcctccgggctggcggagtactg
tcctccggcaaggcggagtactgtcctccgggctggcggagtactgtcctccggcaaggcggagtactgt
cctccgggctggcggagtactgtcctccggcaaggcggagtactgtcctccgggctggcggagtactgtc
ctccggcaaggcggagtactgtcctccgggctggcggagtactgtcctccggcaaggcggagtactgtcc
tccgggctggcggagtactgtcctccggcaaggcggagtactgtcctccgggctggcggagtactgtcct
ccggcaagggtcgagtcgatagccgaagcttaccgaagtatacacttaaattcagtgcacgtttgcttgt
tgagaggaaaggttgtgtgcggacgaatttttttttgaaaaccggtgatagagcctgaaccagaaaagat
aaaagaaggctataccagtgggagtacacaaacagagtaagtttgaatagtaaaaaaaatcatttatgta
aacaataacgtgactgtgcgttaggtcctgttcattggtaccctcgaggtcgacctgcagccaagcttat
gagttaattcaaaccccacggacatgctaagggttaatcaacaatcatatcgctgtctcactcagactca
atacgacactcagaatactattcctttcactcgcacttattgcaagcatacgttaagtggatgtctcttg
ccgacgggaccaccttatgttatttcatcatggtctggccattctcatcgtgagcttccgggtgctcgca
tatctggctctaagacttcgggcccgacgcaaggagtagccgacatatatccgaaataactgcttgtttt
ttttttttaccattattaccatcgtgtttactgtttattgccccctcaaaaagctaatgtaattatattt
gtgccaataaaaacaagatatgacctatagaatacaagtatttccccttcgaacatccccacaagtagac
tttggatttgtcttctaaccaaaagacttacacacctgcataccttacatcaaaaactcgtttatcgcta
cataaaacaccgggatatattttttatatacatacttttcaaatcgcgcgccctcttcataattcacctc
caccacaccacgtttcgtagttgctctttcgctgtctcccacccgctctccgcaacacattcaccttttg
ttcgacgaccttggagcgactgtcgttagttccgcgcgattcggtgcggtatttcacaccgcatatggtg
cactctcagtacaatctgctctgatgccgcatagttaagccagccccgacacccgccaacacccgctgac
gcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgca
tgtgtcagaggttttcaccgtcatcaccgaaacgcgcgagacgaaagggcctcgtgatacgcctattttt
ataggttaatgtcatgataataatggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcgga
acccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaa
tgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttt
tgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcag
ttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccg
aagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgc
cgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcaca
gaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataaca
ctgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatggg
ggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgac
accacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagctt
cccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttcc
ggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactg
gggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaac
gaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactc
atatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgat
aatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatca
aaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctacc
agcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactgacttcagcagagcg
cagataccaaatactgttcttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgc
ctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgg
gttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacag
cccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgc
ttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgaggga
gcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcga
tttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccttcttcttgaactcgggctcggtgc
cagtatacctcaaatggttgtcgtacctctcatggttccgttacgccaacaagggtctgctgattaacca
atgggcggacgtgaagccgggcgaaattagctgcacatcgtcgaacaccacgtgccccagttcgggcaag
gtcatcctggagacgcttaacttctccgccgccgatctgccgctggactacgtgggtctggcc
--Array_of_Hedgehogs_970_000
Content-Description: EPgy2.txt
Content-MD5: pOPzyyw68QmrH/f3FW658A==
>EPgy2 P element, from 5'P end
catgatgaaataacataaggtggtcccgtcgatagccgaagcttaccgaagtatacacttaaattcagtg
cacgtttgcttgttgagaggaaaggttgtgtgcggacgaatttttttttgaaaacattaacccttacgtg
gaataaaaaaaaatgaaatattgcaaattttgctgcaaagctgtgactggagtaaaattaattcacgtgc
cgaagtgtgctattaagagaaaattgtgggagcagagccttgggtgcagccttggtgaaaactcccaaat
ttgtgatacccactttaatgattcgcagtggaaggctgcacctgcaaaaggtcagacatttaaaaggagg
cgactcaacgcagatgccgtacctagtaaagtgatagagcctgaaccagaaaagataaaagaaggctata
ccagtgggagtacacaaacagagtaagtttgaatagtaaaaaaaatcatttatgtaaacaataacgtgac
tgtgcgttaggtcctgttcattgtttaatgaaaataagagcttgagggaaaaaattcgtactttggagta
cgaaatgcgtcgtttagagcagcagccgaattaattctagttccagtgaaatccaagcattttctaaatt
aaatgtattcttattattatagttgttatttttgatatatataaacaacactattatgcccaccattttt
ttgagatgcatctacacaaggaacaaacactggatgtcactttcagttcaaattgtaacgctaatcactc
cgaacaggtcacaaaaaattaccttaaaaagtcataatattaaattagaataaatatagctgtgagggaa
atatatacaaatatattggagcaaataaattgtacatacaaatatttattactaatttctattgagacga
aatgaaccactcggaaccatttgagcgaaccgaatcgcgcggaactaacgacagtcgctccaaggtcgtc
gaacaaaaggtgaatgtgttgcggagagcgggtgggagacagcgaaagagcaactacgaaacgtggtgtg
gtggaggtgaattatgaagagggcgcgcgatttgaaaagtatgtatataaaaaatatatcccggtgtttt
atgtagcgataaacgagtttttgatgtaaggtatgcaggtgtgtaagtcttttggttagaagacaaatcc
aaagtctacttgtggggatgttcgaaggggaaatacttgtattctataggtcatatcttgtttttattgg
cacaaatataattacattagctttttgagggggcaataaacagtaaacacgatggtaataatggtaaaaa
aaaaaacaagcagttatttcggatatatgtcggctactccttgcgtcgggcccgaagtcttagagccaga
tatgcgagcacccggaagctcacgatgagaatggccagacccacgtagtccagcggcagatcggcggcgg
agaagttaagcgtctccaggatgaccttgcccgaactggggcacgtggtgttcgacgatgtgcagctaat
ttcgcccggctccacgtccgcccattggttaatcagcagaccctcgttggcgtaacggaaccatgagagg
tacgacaaccatttgaggtatactggcaccgagcccgagttcaagaagaagccgccaaagagcaggaatg
gtatgataaccggcggacccacagacagcgccatcgaggtcgaggagctggcgcaggatattagatatcc
gaaggacgttgacacattggccaccagagtgaccagcgccaggcagttgaagaagtgcagcactccggcc
cgcagtccgatcatcggataggcaatcgccgtgaagaccagtggcactgtgagaaaaagcggcaattcgg
caatcgttttgcccagaaagtatgtgtcacagcgataaagtcgacttcgggcctccctcataaaaactgg
cagctctgaggtgaacacctaaatcgaatcgattcattagaaagttagtaaattattgaaatgcaaatgt
attctaaacatgacttacatttatcgtggcaaagacgttttgaaaggtcatgttggtcaggaagaggaag
atggctccgttgatattcatcacacccacttgcgtgagttgttggcccaaaaagatgaggccaatcaaga
tggcaaccatctgcaaattaaaatgttactcgcatctcattaatattcgcgagttaaatgaaatttattt
atcttctgcaaaactataaactatacatctcattgaaaaaaactaagaagggtgtggaatcaggcaattc
tatctaaaatctagcgaatttgtttccaagaattgtaagcgttatatcatttgtttccactggaaccact
caccgttgtctgaataagtcgcacttttacgaggagtggttccttgagcaccgacagccaggatcgccac
aggaccgcccggaactgcatgaaccaggtggccttgtaggtgtacccattctccggctgctccagtggct
tctccagatttttggtggccaacaactgctccatatcccgggctactttgctaatggcaaaattgtcgca
tatcttggcgatccgatcacgggactcgatctcccgtccgggcacaacggccaacacctgtacgtaaaag
tccgccggattgtagttggtaggacactgggcacccacgctggataggagttgagatgttatgtaatact
agatacccttaataaacacatcgaactcactaggaaaagaagtcgacggcttcgctgggagtgcccaaga
aagctaccctgccctcggccatcagaaggatcttgtcaaagagctcaaacagctcggaagacggctgatg
aatggtcaggatgacggtcttgcccttctgcgacagcttcttcagcacctggacgacgctgtgggcggta
aaggagtccagtccggaggtgggctcatcgcagatcagaagcggcggatcggttagagcctcggaggcga
atgccagacgcttcctttctccgccggacagacctttcaccctgccgggcacaccgatgatcgtgtgctg
acatttgctgagcgaaagctcctggatcacctgatccacgcgggccactcgctgccgataggtcagatgt
cgtggcatccgcaccatggcttggaaaatcaggtgttccctggccgttagggagccgataaagaggtcat
cctgctggacataggcgcacctggcctgcatctccttggcgtccacaggttggccattgagcagtcgcat
cccggatggcgatacttggatgccctgcggcgatcgaaaggcaagggcattcagcagggtcgtctttccg
gcaccggaactgcccatcacggccaaaagttcgcccggataggccacgccgcaaactgagtttcaaattg
gtaattggaccctttattaagatttcacacagatcagccgactgcgaatagaaactcaccgttcttgagc
aaatgtttcctgggcgccggtatgtgtcgctcgttgcagaatagtccgcgtgtccggttgaccagctgcc
gccatccggagcccggctgattgaccgccccaaagatgtccatattgtgccaggcataggtgaggttctc
ggctagttggccgctccctgaaccggagtcctccggcggactgggtggccggagcgtgccgtagtttttg
gcctgcccgaagccctggttaatgcagctctgcgaagccgctccgctgtcaccctgcaatgataggggat
ctcaaatatcaactacaagcgttatgctcatctaaccccgaacaaaaagtaccccgaagtatcctacgaa
gtaggtttatacttttatttattttttgtgcatctaggatcagcttaaaatatctggttgttatattttt
tgtaaaaaagaatatagtcgaaaatgaatgcctttagatgtcttgatcatgatatgatctcaaaaattgt
cttatatagcgagaacagctaccagaataatctgtttcgtgtcactatttgtttgtgcaattgcggtttg
ggatttttgtgggtcgcagttctcacgccgcagacaatttgatgttgcaatcgcagttcctatagatcaa
gtgaacttaagatgtatgcacatgtactactcacattgttcagatgctcggcagatgggtgtttgctgcc
tccgcgaattaatagctcctgatcctcttggcccattgccgggatttttcacactttcccctgcttaccc
acccaaaaccaatcaccaccccaatcactcaaaaaacaaacaaaaataagaagcgagaggagttttggca
cagcactttgtgtttaattgatggcgtaaaccgcttggagcttcgtcacgaaaccgctgacaaaatgcaa
ctgaaggcggacattgacgctacgtaacgctacaaacggtggcgaaagagatagcggacgcagcggcgaa
agagacggcgatatttctgtggacagagaaggaggcaaacagcgctgactttgagtggaatgtcattttg
agtgagaggtaatcgaaagaacctggtacatcaaatacccttggatcgaagtaaatttaaaactgatcag
ataagttcaatgatatccagtgcagtaaaaaaaaaaaatgttttttttatctactttccgcaaaaatggg
ttttattaacttacatacatactagaattcggtacccgcccggggatcagatccgcggccgctctagaac
tagtggatcccccgggctgcaggaattcgatatcaagcttatcgataccgtcgacctgcaggtcaacgga
tcctaccctaccaccaacaaagtctttttgtttgccccgtctgaagtctggctccgatcaaaagcaaaaa
tttcaattacagtcgaatctcaagcgaccaggcgatctcaaattgagcgattaagttgaactttccctgc
acccaaacaagatcttgtgcatagctttatcaacctactttcttgcctggtagtagtactccagaaaatg
tacagttgttgatatatttagaatttatgcatacttacattttttccgctttttccgctcaagaaaattg
cgtaaactcttaaccttcaaaaaaaagttgatttattgttattttttgcttaacataactagataaagta
ttgatttgccacttgctcatacgtcatgtggtttttttaaccgctgttgccctatgttgttgtctttaaa
tattctttacatcaatcgagtgtgtgagaatatacccaagtaccgtgtgtaggattatgttaaccttgat
gctgatgatgccaccacccagattggcgggcattcacataagtttttaacccaccatcctggccacccgg
ttgggcgggttgatgggtgggaaatagatggggaccacttgtctcgtaatttatgccgttgtgctggttg
aaaatataggcctttggcacctccactcctgcaggacccacagaatttgtagagacactaatactggaga
ctacattgcctgaattggcgggcaaataagtgcgacttggaggaggcggcgaggaagcccagctggtctg
aggtttctgtggcaagacaggacgatattgtttcgtatataacggtggacccattggcaaaacggcttgt
tttggtattgaaacggtatttggcggcccataggcgttattcctcaaatcacacacagtattctcaatta
aagtggccaagggagccgtgtaaattcggaaattagtatctgaataatccaagtcagacagcaagaaaac
gggcatcctatcggatagaacccaaacgtttttgttctcatcaattttcacatcggccggaaaaactaag
ccaacgtcatcgcgatccacaatgccatgaaattgcggtgagtacggcattgatgagtgccagcaaccca
ctgcattttgatctattaaattgaacagctcaattccatcatcgctcatcacacgtgaagtggtatggga
gtttggaccccgttcatctaaggcaacaaagtcatgatagctatcttccgtcctggtttcatccctcaaa
atcctcgtggatacggcaaattgtcgatgacttgctaacggactaaagtacagggtacgataaccatccg
atcgaatgggcgaaagggacataccaaatataccctcctcgccccattggaagttaataccagcgacatt
gaaatcgcccctcaatggatcggggaaaaaatacgaatgtgccgagaatctccaggacttgttcagttcc
caggagtaagcaatcaagccgtatcccaattcatcggcaaaataggcatatgcatcatcgcaatttttgc
ctatatccacggcaatgttagctatgaaagtatttggatttgtgtccacgccaggtagctcgtatctccg
aattcgcgtatccgtggtcaagtcaaagacatttaccgcataggggcacggattagtggtggtattgccg
atgcccacggttccagtgtccaaaacccacagccgaccacactcatccactttaatgcggtaggcagtgg
taatactgttggcgcaatctccagctgtatttgagcgccaatctggatacggaattagctccggtgaacc
cgtcaaactgcggtccatgtttatataggtcagagtggccggaatcccatcacgccagcggggaacagtg
acgaataaccgattgccaaagtgttcgactccaacaggtagagcattttgcggaatataatctccactag
ccagagcttggtcctttagtcgggtattcgggaaagcaaagtccagctggctccaactatatcgctcctg
aagtttgtaagcagcccaagacggcgtcaccaaggtgatcagggtcacaaggatccaccctttgtcctgg
aacattgcacttagctctaagctgacaatcactcttaacacttaagccaaagtgtgttggcaataaaaaa
aaatatatttgtttatatatgttcagctatagggggttcttctctagccttcggctgtgtgatatttttt
taatcctcttctgtggaccgtggcgcggtaacgactggtggccataatatgtcggccgcgttttatatga
aggtttttttctccgaagacgaagacaggccaatgaaaatgaaaacgaaggcgcgcgccaacttcggtgg
ttttagtgattcgggtggttcagtgttcgggtaatcaggtggcttatgctgttcccatagatcggccaac
tttgcgttttgtcttccatgattgattttcacgcatgtttaataaatacactcgaatcccccaatttaat
gagtcagacatcacctttcgctgggtttggtatgatttttggccttcatcgacatttaaaactgcgctga
tttgtgccgaaatacctttcggtaccctaaaagattaagacctattttaaagttgtgactactatttatt
taattttagggactttatttgacgatctaatttcaaacaagtaaattgtaaggccaggaggctcgtgcat
agaatgcattgctctccaaatttctgaaaatctgaaagcttaatacatgacagttgtgttctgagaactg
tgttcatctttatcggcgactgcaaaaaatgttctaatatgattttttagttcaattcattaaaaaaatc
aaatcaatattatatgaaatacatgcagacaaaaatccagaaatatcaaataaatcgttattataacgtg
tactatgtagagtatttaaagcaggtgtttccttgtatactattaaattggaactcgtgctcgtttcaat
tgcttaggattggatttcgattgggcgtcaccgatgattcacaattcactgagggtcaaatatttggttt
ccgctagttattggcaggtgattttgagcatacatatacataaatataattgagtgttacataaaatttc
aaaaaggaacgaaagcgcttttaggtaattccactagctgttatttaaaatattgtggtccctgaaaatc
aactggatgttgggggtgccctaagtgggtaatgctctaatgaccatcgccgcaagtaaatgttaaaaat
tcgaaccgaaagcgatcgcatatatctcttggcccaaatgcacctgcttgctaaagaaaaaattgcaaaa
cttaagtaaagtaaacaaccagcgacacgcataataaacatgcaaatgggtaaaaatacgttttacgacc
tccgcaggtaaagtggtattgcattaatcctcaataatctacactttaaataaggaaaacctctcaaaat
attaaaatacaaattaaagaatgccgtttaaatttaatcttatttcttcggatgtctaaaaacaatttca
aagctaatgtaaccattactgctattgaattggtgtagtttttggactctatatcatttatgcaatgtct
attttttcttagaagtggttcaacttgatcccttgcccgcattctaaatcattgcacccacagaaaaaat
aaacaaacacggtgtggcaatcgaaaaatatgttgcacaaaattaccggcaatgctgctcgaatttattg
cgatcggcaggcttattaaaagacacacttgaaataaccagtacagttttattaattaattgtgtgtgtt
gtgtaatgaaatggtgtatttactaaaaacgcaactaatgatgcgatcgctgcagccgttgaacctataa
tttttttcgaattgacttgtttcaagtttttgcttaattgtgtgggggaatgctgcgtttgtctgtttgg
ttactgtttttctttttcgcttttcagagcttgcggcaatgatttatacgcacttttcccaataattgaa
acaagttttctgccccagcacaaaacaaaacaacatcagcggagcgcgctaagtggtcaaaaaaagcttc
cacaaatcagatctctttggccataattgaccaacgcttatgcaaccgaccggcaacattcgacattatt
tacacacaggatcacttcaacttgggcggaaagcaaagccgaataccgcatgacattgcccgacccgtgt
ttcatgttttattttccttattccccacttatggcagcctggacattatctccctccctcccctcccccc
tcctacactaatttggaaatccatcgtgactgcaaaacgaaaaacccgttgctcaacttctctaacataa
gcggtacacagcattttccgttttgtgttatatatttttgtgttttttttttttcaagttcaatgtccat
ggccctgaccgccgggtcccctcctttcgagctgggattatttggcatggccaaattcggaaggccgcga
tcacttcccgcttacacacaattgcacatgtccccatgacatgcactgcgctcaaactattagtacccct
tagtaacttaagccaaattaaaaataagttcattaatataaataaaatagtcaatttccactgtcccgat
tgaatagagaaactttatttgtaaattttaaatgtcgccaaggaactcgctagatatactaaaccaaaac
cgattgtagctcccgaaatcgcaaccataagtggaataattagatgccaacaaaggctttgtttgcttac
tgaataaaggttgtcgtaatttgatttctgtgtaatcttgcacgaaacggaatcattacctcagacataa
tttgcctgcaattgcggtggtgcacttgtctcgtgcaagcgaaaaaatcagtgctcgaaaaagcttagtg
gatatactcgtacatatactatgaatcgtttagaggggtaggtaaatcagcgggctgcgttcgaaattta
tgagtgactctgcgacgtatttatcttaagccatcgtcagttgcatattttatggctattttaaaccact
caatgtggtaatcgggcgataatcatttaatagtcgacctcgagggggggccgctctagccccccctcga
atgttctctctcttctcttctctctctctttctcgaatgttctctctcttctcttctctctctctttctc
gaggtcatcaagcttaggcctccaaggcggagtactgtcctccgggctggcggagtactgtcctccggca
aggcggagtactgtcctccgggctggcggagtactgtcctccggcaaggcggagtactgtcctccgggct
ggcggagtactgtcctccggcaaggcggagtactgtcctccgggctggcggagtactgtcctccggcaag
gcggagtactgtcctccgggctggcggagtactgtcctccggcaaggcggagtactgtcctccgggctgg
cggagtactgtcctccggcaaggcggagtactgtcctccgggctggcggagtactgtcctccggcaaggg
tcgagtcgatagccgaagcttaccgaagtatacacttaaattcagtgcacgtttgcttgttgagaggaaa
ggttgtgtgcggacgaatttttttttgaaaaccggtgatagagcctgaaccagaaaagataaaagaaggc
tataccagtgggagtacacaaacagagtaagtttgaatagtaaaaaaaatcatttatgtaaacaataacg
tgactgtgcgttaggtcctgttcattggtaccctcgaggtcgacctgcagccaagcttatgagttaattc
aaaccccacggacatgctaagggttaatcaacaatcatatcgctgtctcactcagactcaatacgacact
cagaatactattcctttcactcgcacttattgcaagcatacgttaagtggatgtctcttgccgacgggac
caccttatgttatttcatcatg
--Array_of_Hedgehogs_970_000
Content-Description: NoName
Content-MD5: HCi0qs2rF+7Y4svz+WbtjA==
--
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Robert W. Levis, Ph.D.
Carnegie Institution of Washington
Department of Embryology
115 West University Parkway
Baltimore, MD 21210, U.S.A.
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