Subject: Re: FlyBase query: abstract 1049B
Dear Dr. Millburn,
In release 3 of genome annotation, multiple is CG31719 and yumo is CG6187.
CG31719 is merged by CG13143 and CG7475 of the release 2.
multiple and yumo may correspond to 'Scim16', Sensitized chromosome
inheritance modifiers .
Sequence analysis off ends of P{SUPor-P} in Scim insertion mutant places
'Scim16' near/in 'CG13143' or 'CG6187'. (Genetics, 2001, 157:1623 ).
However, the insertion site is not clear.
By sequence analysis, Multiple and Yumo contain the pseudouridine synthase 2
domain which is shared by the RluA-family pseudouridine synthase of E.
coli.
Therefore, it may be more appropriate to rename multiple as RluA-1 and yumo
as RluA-2.
Thanks,
CT
Cheng-Ting Chien, Ph. D.
Associate Research Fellow
Institute of Molecular Biology
Academia Sinica
Taipei, Taiwan
\----- Original Message \-----
Sent: Tuesday, March 18, 2003  9:32  PM
Subject: FlyBase query: abstract 1049B
> Dear Dr. Chien,
>
> I am curating your abstract from the addendum of the 44th
> (Chicago) Annual Drosophila Research Conference, for FlyBase.
>
> I am writing in connection with your abstract:
>
> 1049B: multiple encodes an RluA pseudouridine synthase-like protein in
> multiple dendritic neurons in the Drosophila peripheral nervous
> system.
>
> You mention 2 gene symbols that I think are new to FlyBase: 'yumo' and
> 'multiple'.
>
> 1. yumo
>
> Do you know which of the Genome Project CG annotations yumo corresponds
> to? All the CGs have corresponding gene records in FlyBase already and
> we don't like to make duplicate records for what is actually the same
> gene unless we can't avoid it.
>
> 2. multiple
>
> There is already a gene with the name 'multiple' (short symbol: mul) in
> FlyBase, but this gene maps to chromosome 1, at 1B-1C.
>
> Your 'multiple' gene maps near to the E7-2-36 enhancer trap insertion
> line, which according to Uemura et al., 1989, Cell 58: 349--360 maps to
> chromosome 2.
>
> Please could you confirm that your 'multiple' gene is different from
> the one in FlyBase.
>
> If it is different, could you tell me which CG it corresponds to. Also,
> since the name 'multiple' is already taken by the gene on chromosome 1,
> can you suggest a different name for your 'multiple' gene,
>
> thanks,
>
> Gillian
>
> \--------------------------------------------------------------
> Gillian Millburn.
>
> FlyBase (Cambridge),
>
> FlyBase: http://fly.ebi.ac. uk:7081 /
> \--------------------------------------------------------------