Subject: FlyBase query
Dear Dr. Acharya,
I am curating your paper for FlyBase:
Acharya et al., 2003, Science 299(5613): 1740--1743
and I have a question about the UAS-ceramidase construct you made.
I would be grateful if you could tell me which of the CG gene
predictions your 'ceramidase' corresponds to so that I make sure I put
the construct under the correct gene in the database and can rename it
to 'CDase'.
In the 'Supporting Online Material' you state that it corresponds to
'EST SD007768', however, there are too many numbers in this EST number
\- it should only have 5 numbers after the 'SD'.
Did you mean 'EST SD07768' ?
thanks,
Gillian
\--------------------------------------------------------------
Gillian Millburn.
FlyBase (Cambridge),
FlyBase: http://fly.ebi.ac. uk:7081 /
\--------------------------------------------------------------
Subject: Re: FlyBase query
Hello Dr.Millburn,
The CDase we refer to is CG1471. The est we used is SD07768.
Thank you,
Jairaj Acharya
>Dear Dr. Acharya,
>
>I am curating your paper for FlyBase:
>
>Acharya et al., 2003, Science 299(5613): 1740--1743
>
>and I have a question about the UAS-ceramidase construct you made.
>
>I would be grateful if you could tell me which of the CG gene
>predictions your 'ceramidase' corresponds to so that I make sure I put
>the construct under the correct gene in the database and can rename it
>to 'CDase'.
>
>In the 'Supporting Online Material' you state that it corresponds to
>'EST SD007768', however, there are too many numbers in this EST number
>- it should only have 5 numbers after the 'SD'.
>
>Did you mean 'EST SD07768' ?
>
>thanks,
>
>Gillian
>
>--------------------------------------------------------------
>Gillian Millburn.
>
>FlyBase (Cambridge),
>
>FlyBase: http://fly.ebi.ac. uk:7081 /
>--------------------------------------------------------------
\--
Jairaj Acharya
Principal Investigator
National Cancer Institute, Frederick
Room.22-6, Building 560
1050 Boyle Street, Frederick, MD 21702
USA