Subject: personal communication to fly base
We are writing to you about the phtf gene (Gene phtf : FBgn0028579).
we would like to add an expression data about phtf expression in drosophila
testis. We have shown that the level of accumulation of d-phtf transcript
was almost imperceptible in germ line-les compared to wild-type males
indicating that expression of the d-phtf gene in Drosophila is essentially
restricted to the male germinal cells.The canton S stock of Drosophila
melanogaster was used. Germ line-less flies were obtained by crossing
tud1bw sp/tud1 bw sp virgin females with wild-type males of the Canton S
stock.
The figure represents a northern blot of RNAs isolated from wild-type (wt)
and germ line-less tudor (Tu) adult male flies probed with a d-phtf probe.
Levels of RNA were verified by hybridization with a control probe specific
for the rpL17A gene
Citation from C. Yanicostas and N. Raich