Isolation and characterization of Df(2L)BSC30
Jennifer Deal and Kevin Cook
Bloomington Drosophila Stock Center, Indiana University
Df(2L)BSC30 was isolated as a P transposase-induced male recombination event involving P{PZ}l(2)0143301433 and P{lacW}l(2)k00302k00302. The deletion was isolated as a dp+-cn+ recombinant chromosome from the cross dpov1 cn1 bw1 females x P{PZ}l(2)0143301433 cn1/dpov1 P{lacW}l(2)k00302k00302; TMS, P{Delta2-3}99B males. The miniwhite marker from P{lacW}l(2)k00302k00302 was disrupted or deleted based on eye color upon outcrossing to w- flies. Polytene chromosome squashes showed the breakpoints 34A3;34B7-9. Df(2L)BSC30 failed to complement Nnp-1k07826 and l(2)k05911k05911, but complemented the visible phenotype of nub2.