Subject: Re: FlyBase Query (cy1823b) Dear Chihiro, The GS6 is a new version of Gene Search misexpression vector. As in the first version of GS vector described in Toba et al., (1999), it contains two copies of UAS/hsp70 module. One copy is located near 5'P end, so that it would misexpress the vector-flanking genes upon GAL4 activation, whereas the other one is followed by GFP/SV40 trailer sequence, thus it would simply mark the GAL4-expressing cells. The map of GS6 vector is available at the following URL. http://www.comp.metro-u.ac.jp/%7Eatsugyou/gs/Methods/Vectors/GSV6/GSV6.html Best wishes, Toshiro At 2:54 PM \+0100 03.4.7, Chihiro Yamada wrote: Dear Dr Aigaki, I am currently curating your paper for FlyBase: Igaki et al., 2002, EMBO J. 21(12): 3009--3018 I have a question I was hoping you could answer for me. GS6 === You describe on page 3011 the GS6 vector, that was used to create the GS9830 allele. We don't have this particular version of GS in our records. Could you give me some molecular details about this transposon? What marker does it carry? Any new information you give us will be curated as a personal communication from you to FlyBase, if thats OK with you Best wishes, Chihiro \---------------------------------------------------------------------- Chihiro Yamada. FlyBase (Cambridge), \---------------------------------------------------------------------- Curator comment: The following is text taken from the website (http://www.comp.metro-u.ac.jp/%7Eatsugyou/gs/Methods/Vectors/GSV6/GSV6.html) GSV6 6836 bp GSV6 contains two copies of UAS enhancer adjacent to a core promoter at 5'P end and upstream of green fluorescent protein (GFP) and SV40 trailer sequence inserted near the 3'P end. Therefore, misexpression of the vector flanking sequence occurs 5'P site only, and GFP will be expressed under control of GAL4.
