Subject: Re: FlyBase Query (cy1880) Hi Chihiro, .. We used the tubulin alpha 1 promoter from the Tubulin>CD2>hh construct (Basler & Struhl 1994). I don't know from which tubulin locus it was derived. This was cloned as an EcoRI-KpnI fragment into Casper 4, followed by EGFP (clontech EGFP-N1) as a KpnI-NotI fragment. This construct is described first in Brennecke et al 2003. Steve >Dear Dr Cohen, > >I am currently curating your paper for FlyBase: > >Brennecke et al., 2003, Cell 113(1): 25--36 > >I have a quick question I was hoping you could answer for me. > >Tub-EGFP >======== >You use a Tub-EGFP as a starting point for your bantam sensor >construct. I'm not sure whether we have this construct in our records >yet. > >Where did you get it from? Has it been published before, if so what was >it called? Can you tell me which Tubulin promoter was used to drive >expression of EGFP? > >αTub67C >αTub84B >αTub84D >αTub85E > >Best wishes, > >Chihiro > >---------------------------------------------------------------------- >Chihiro Yamada. > >FlyBase (Cambridge). >---------------------------------------------------------------------- \-- Stephen Cohen Developmental Biology Programme EMBL Meyerhofstr 1 69117 Heidelberg, Germany
