Gene or accession: Gr22bP
Release: 3
Missed gene
Gene cDNA sequence:
>Gr22bP
ATGTTTGGTTCAAGTCGCGAAATTCGTCCTTATTTGGCAAGACAGATGCTGAAAACCACACTTTATGGATCCTGGCTACT
CGGTATATTCCCCTTTACCCTCGACTCCGGAAAAAGAATACGTCAACTGAGACGATCCCGCTGCCTAACTTTGTACGGCC
TGGTACTTAATTACTTCCTTATATTTACTCTGATTTGATTGGCTTTTGAATACCGGAAACATAAACTAGAAGCCTTTAAG
CGAAATCCTGTGCTGGAGATGATAAATGTAGTAATTGGAATTATAAACGTTCTTTCAGCCCTGATTGTACACTTTATGAA
CTTTTGGGGCAGCAGAAAAGTTGGTGAGATATGCAACGAACTTTTAATTCTTGAGTATCAAGACTTTGAAGGCCTTAATG
GGAGAAATTGCCCAAATTTCAACTGCTTTGTGATTCAAAAGTGTTTGACAATATTGGGCCAGCTGTTATCTTTCTTCACG
CTCAACTTCGCACTTCCGGGCCTTGAGTTTCATATATGTCTAGTACTTCTGAGCTGCCTGATGGAGTTTTCGCTCAATTT
GAACATTATGCACTACCATGTCGGTGTCCTGTTAATCTATCGCTATGTATGGCTGATAAATGAACAACTGAAGGACCTAG
TGAGCCAATTAAAACTGAATCCTGAGACAGACTTCTCCAGAATACATCAGTTTCTATCCTTATACAAACGCCTTCTAGAG
CTAAATAGAAAATTGGTGATCGCCTACGAATACCAAATGACCCTATTTATAATAGCTCAGCTATCTGGAAACATCGTGGT
CATTTATTTCCTCATTGTATATGGATTAAGTATGCGTACTTATTCTATCTTCTTGGTGGCATTCCCAAATTCCCTGTTAA
TAAACATTTGGGATTTCTGGTTGTGCATTGCCGCATGCGATCTTACCGAAAAGGCTGGTGACGAAACCGCGATAATCCTA
AAAATATTTTCTGACCTCGAGCACAGAGATGATAAGCTAGAAATGAGTGTTAACGAGTTCGCTTGGCTTTGCAGTCACCG
GAAGTTCCGATTTCAACTGTGTGGACTATTTTCTATGAACTGTAGAATGGGCTTCAAAATGATCATAACCACTTTCCTGT
ACTTAGTATATTTAGTTCAGTTTGATTATATGAACTTGTAA
Protein sequence:
>Gr22bP
MFGSSREIRPYLARQMLKTTLYGSWLLGIFPFTLDSGKRIRQLRRSRCLTLYGLVLNYFLIFTLIZLAFEYRKHKLEAFK
RNPVLEMINVVIGIINVLSALIVHFMNFWGSRKVGEICNELLILEYQDFEGLNGRNCPNFNCFVIQKCLTILGQLLSFFT
LNFALPGLEFHICLVLLSCLMEFSLNLNIMHYHVGVLLIYRYVWLINEQLKDLVSQLKLNPETDFSRIHQFLSLYKRLLE
LNRKLVIAYEYQMTLFIIAQLSGNIVVIYFLIVYGLSMRTYSIFLVAFPNSLLINIWDFWLCIAACDLTEKAGDETAIIL
KIFSDLEHRDDKLEMSVNEFAWLCSHRKFRFQLCGLFSMNCRMGFKMIITTFLYLVYLVQFDYMNL
Comments: This gene was recognized as a young pseudogene by Dunipace et al
(2001), but Scott et al (2001) avoided the stop codon in the first exon by
starting their annotation later (which is unsatisfactory because then the
protein doesn't have a TM1 region comparable to Gr22a, c, e, and f. I think it
would be good to somehow annotate this in FlyBase as Gr22bP. We have amplified
and sequenced the first exon from an Oregon R strain and find that the stop
codon is a CGA encoding arginine there. So like Or85eP this appears to be a
polymorphic pseudogene in the species.