Gene or accession: CG17849
Release: 3
Gene annotation error
Gene CG17849 has incorrect exon/intron structure or translation start site.
Gene cDNA sequence:
>Or46aA
ATGAGCAAAGGAGTAGAAATCTTTTACAAGGGCCAGAAGGCATTCTTGAACATCCTCTCGTTGTGGCCTCAGATAGAAC
GCCGGTGGAGAATCATCCACCAGGTGAACTATGTCCACGTAATTGTGTTTTGGGTGCTGCTCTTTGATCTCCTCTTGGT
GCTCCATGTGATGGCTAATTTGAGCTACATGTCCGAGGTTGTGAAAGCCATCTTTATCCTGGCCACCAGTGCAGGGCAC
ACCACCAAGCTGCTGTCCATAAAGGCGAACAATGTGCAGATGGAGGAGCTCTTTAGGAGATTGGATAACGAAGAGTTCC
GTCCTAGAGGCGCCAACGAAGAGTTGATCTTTGCAGCAGCCTGTGAAAGAAGTAGGAAGCTTCGGGACTTCTATGGAGC
GCTTTCGTTTGCCGCCTTGAGCATGATTCTCATACCCCAGTTCGCCTTGGACTGGTCCCACCTTCCGCTCAAAACATAC
AATCCGCTTGGCGAGAATACCGGCTCACCTGCTTATTGGCTCCTCTACTGCTATCAGTGTCTGGCCTTGTCCGTATCCT
GCATCACCAACATAGGATTCGACTCACTCTGCTCCTCACTGTTCATCTTCCTCAAGTGCCAGCTGGACATTCTGGCCGT
GCGACTGGACAAGATCGGTCGGTTAATCACTACTTCTGGTGGCACTGTGGAACAGCAACTTAAGGAAAATATCCGCTAT
CACATGACCATCGTTGAACTGTCGAAAACCGTGGAGCGTCTACTTTGCAAGCCGATTTCGGTGCAGATCTTCTGCTCGG
TTTTGGTGCTGACTGCCAATTTCTATGCCATTGCTGTGTTATCTGACGAGAGGCTGGAGCTCTTTAAGTATGTGACCTA
TCAGGCGTGCATGTTGATTCAGATTTTTATATTGTGCTACTATGCCGGTGAGGTAACCCAGCGCAGCCTGGACCTTCCG
CACGAGCTGTACAAGACCTCCTGGGTGGACTGGGACTACAGGAGCCGAAGGATTGCGCTCCTCTTTATGCAACGCCTTC
ACTCGACCTTGAGGATTAGGACACTTAATCCAAGTCTTGGTTTTGACTTAATGCTCTTCAGCTCGATCGTCAACTGCTC
CTACAGCTACTTCGCACTGCTGAAGCGCGTCAACAGTTAA
>Or46aB
ATGGTTACGGAGGACTTTTATAAGTACCAGGTGTGGTACTTCCAAATCCTTGGTGTTTGGCAGCTCCCCACTTGGGCCG
CAGACCACCAGCGTCGTTTTCAGTCCATGAGGTTTGGCTTCATCCTGGTCATCCTGTTCATCATGCTGCTGCTTTTCTC
CTTCGAAATGTTGAACAACATTTCCCAAGTTAGGGAGATCCTAAAGGTATTCTTCATGTTCGCCACGGAAATATCCTGC
ATGGCCAAATTATTGCATTTGAAGTTGAAGAGCCGCAAACTCGCTGGCTTGGTTGATGCGATGTTGTCCCCAGAGTTCG
GCGTTAAAAGTGAACAGGAAATGCAGATGCTGGAATTGGATAGAGTGGCGGTTGTCCGCATGAGGAACTCCTACGGCAT
CATGTCCCTGGGCGCGGCTTCCCTGATCCTTATAGTTCCCTGTTTCGACAACTTTGGCGAGCTACCACTGGCCATGTTG
GAGGTATGCAGCATCGAGGGATGGATCTGCTATTGGTCGCAGTACCTTTTCCACTCGATTTGCCTGCTGCCCACTTGTG
TGCTGAATATAACCTACGACTCGGTGGCCTACTCGTTGCTCTGTTTCTTGAAGGTTCAGCTACAAATGCTGGTCCTGCG
ATTAGAAAAGTTGGGTCCTGTGATCGAACCCCAGGATAATGAGAAAATCGCAATGGAACTGCGTGAGTGTGCCGCCTAC
TACAACAGGATTGTTCGTTTCAAGGACCTGGTGGAGCTGTTCATAAAGGGGCCAGGATCTGTGCAGCTCATGTGTTCTG
TTCTGGTGCTGGTGTCCAACCTGTACGACATGTCCACCATGTCCATTGCAAACGGCGATGCCATCTTTATGCTCAAGAC
CTGTATCTATCAGCTGGTGATGCTCTGGCAGATCTTCATCATTTGCTACGCCTCCAACGAGGTAACTGTCCAGAGCTCT
AGGTTGTGTCACAGCATCTACAGCTCCCAATGGACGGGATGGAACAGGGCAAACCGCCGGATTGTCCTTCTCATGATGC
AGCGCTTTAATTCCCCGATGCTCCTGAGCACCTTTAACCCCACCTTTGCTTTCAGCTTGGAGGCCTTTGGTTCTATCGT
CAACTGCTCCTACAGCTACTTCGCACTGCTGAAGCGCGTCAACAGTTAA
Protein sequence:
>Or46aA
MSKGVEIFYKGQKAFLNILSLWPQIERRWRIIHQVNYVHVIVFWVLLFDLLLVLHVMANLSYMSEVVKAIFILATSAGHT
TKLLSIKANNVQMEELFRRLDNEEFRPRGANEELIFAAACERSRKLRDFYGALSFAALSMILIPQFALDWSHLPLKTYNP
LGENTGSPAYWLLYCYQCLALSVSCITNIGFDSLCSSLFIFLKCQLDILAVRLDKIGRLITTSGGTVEQQLKENIRYHMT
IVELSKTVERLLCKPISVQIFCSVLVLTANFYAIAVLSDERLELFKYVTYQACMLIQIFILCYYAGEVTQRSLDLPHELY
KTSWVDWDYRSRRIALLFMQRLHSTLRIRTLNPSLGFDLMLFSSIVNCSYSYFALLKRVNS
>Or46aB
MVTEDFYKYQVWYFQILGVWQLPTWAADHQRRFQSMRFGFILVILFIMLLLFSFEMLNNISQVREILKVFFMFATEISCM
AKLLHLKLKSRKLAGLVDAMLSPEFGVKSEQEMQMLELDRVAVVRMRNSYGIMSLGAASLILIVPCFDNFGELPLAMLEV
CSIEGWICYWSQYLFHSICLLPTCVLNITYDSVAYSLLCFLKVQLQMLVLRLEKLGPVIEPQDNEKIAMELRECAAYYNR
IVRFKDLVELFIKGPGSVQLMCSVLVLVSNLYDMSTMSIANGDAIFMLKTCIYQLVMLWQIFIICYASNEVTVQSSRLCH
SIYSSQWTGWNRANRRIVLLMMQRFNSPMLLSTFNPTFAFSLEAFGSIVNCSYSYFALLKRVNS
Comments: There's a complicated problem with the current Or46a/b gene(s). Like
several of the Grs, we now recognize that they are alternatively spliced,
starting with different promoters and first exons and sharing only the final
short exon. So there is one gene, Or46a, with two alternatively transcribed
transcripts, PA, and PB. Thus the current annotation CG17849-PA should have
the final exon added, and a PB transcript added for the current 46b. CG17848
should be dropped. My cDNAs and proteins are above \- they are confirmed by
the D. pseudoobscura comparison and by RT/PCR from antennae by Coral Warr
(ex-postdoc in John Carlson's lab now in Australia and co-author on our
manuscript). We call these proteins Or46aA/B.