Gorchakov, A.A., Demakov, S.A., Shvarts, Y.B. (2003). Construction of pFRT, a convenient Drosophila transformation vector with the functional FRT site. Molek. Biol., Moscow 37(5): 820--824.
FlyBase ID
FBrf0167915
Publication Type
Research paper
Abstract
Modification of Drosophila transformation vector pCaSper3 with the P element was used to construct a new vector, pFRT. The vector contains two tandem FRT sites flanked with several unique restriction sites and separated by a polylinker of five restriction sites, and allows easy cloning of DNA fragments between or close to the FRT sites. FRT-mediated excision of DNA sequences cloned between the FRT sites was demonstrated in vivo. The vector was proposed for molecular genetic studies of the position effect variegation, structural and molecular organization of Drosophila polytene chromosomes, etc.
