Subject: Re: AP-2 mutations
Dear Beverly,
Your guesses were correct!
Thanks,
Pam Mitchell
>Dear Dr. Mitchell,
>
>I am a curator for FlyBase and am annotating the molecular basis of the
>AP-2 mutations from your 2001 Monge et. al. Development paper. This
>type of information should start appearing soon in the annotation
>reports. I didn't have quite enough information to locate the sites of
>the AP-29 (GG to AG change creating a new splice acceptor in intron
>2) and AP-23 (TG to AG change in intron 1, creating a new splice
>acceptor) mutations.
>
>Could you send me some sequence surrounding the site of the mutations
>so I can locate them accurately in the genome?
>
>For AP-29, I am guessing that it is the most 3' GG in intron 2
>
>wt AGATATCC GG CTACTTGCA
>mutant AGATATCC AG CTACTTGCA
>
>For AP-23, is it also the one closest to the splice acceptor?
>
>wt TTCCGCT TG TCTTCCCC
>mutant TTCCGCT AG TCTTCCCC
>
>Thanks for your assistance,
>
>Sincerely,
>Beverley Matthews
>FlyBase-Harvard
Pamela J. Mitchell, Ph.D.
Associate Professor
Department of Biochemistry & Molecular Biology
Pennsylvania State University
332 South Frear
University Park, PA 16802