Personal communication from Robert Levis, 28 September 2001 1) BG02205 The available data suggest that this is a single insertion, since the 3' and 5' flanks have an 8 bp target site duplication and map to the same site in the prototype 1360/hoppel element. Guochun's software called it multiple, meaning that it hit multiple sites in the genome (i.e. it is repetitive, not a multiple insert). While most of both flanks is repetitive, there is a region of the 5' flank from 270 to ~410 that hits a single scaffold segment, AE003569. It was on this basis that I mapped it to 19F. This might be incorrect if this apparently unique segment is actually repeated elsewhere in the portion of the genome that is not represented in the whole genome assembly. 2) BG02303 The 5' and 3' flanking sequence reads for this insertion map 3.4kb apart. From the available data it cannot be determined whether this is two insertions or a single insertion associated with a deletion. 3) BG02678 The 5' flanking sequence aligns to the blastopia repetitive element, while the 3' flanking sequence maps to a single site in 2R-57C. The absence of a target site duplication suggests two insertions (or an insertion associated with a deletion).