Subject: Exelixis cloning vectors
The following information was provided by Annette Parks of Exelixis, Inc.
(5/04).
Exelixis constructed a variety of vectors which are all variations on
P{Express}:
P{Express-UAS} (FBtp0012992)
P{Express-glass} (FBtp0012991)
P{Express-vgMQ}
P{Express-vgM}
P{Express-ey3.5}
P{Express-ey1x}
P{Express-ey4x}
P{Express-sev3x}
P{Express-sev1x}
P{Express-elav}
P{Express-FRT.2.1}
P{Express-FRT.2.2}
1. P{Express-vgMQ}
Kim et al., 1996 (Nature  382:133-138 ; FBrf0088295) discusses the
identification of two enhancers at the vestigial (FBgn0003975) gene. The
'quadrant' enhancer drives expression in the wing blade and the 'margin'
enhancer drives expression at the dorsal/ventral boundary. Kim et al.
combined the Q and M enhancers into a single contiguous fragment. This MQ
element is what Exelixis cloned into pP{Express} to generate P{Express-vgMQ}.
2. P{Express-vgM}
A similar construct similar to P{Express-vgMQ}, but carrying only the M
enhancer.
3. P{Express-ey3.5}
The DNA fragment driving expression in the pattern of the eyeless
(FBgn0005558) gene is a 3.5 kb Kpn fragment described in Halder et al. 1998
(Development 125: 2181-2191; FBrf0103272).
4. P{Express-ey1x}
Carries one copy of the 200 bp ey enhancer PCR amplified with Kpn/Bam ends.
5. P{Express-ey4x}
Carries four copies of the eyeless enhancer.
6. P{Express-sev3x} and P{Express-sev1x}
These two vectors carry either three copies or one copy of the sevenless
(FBgn0003366) enhancer. The MacVector file gives the sequence of the
P{Express-sev3x} enhancer.
7. P{Express-elav}
Carries sequences from the elav (FBgn0000570) gene to drive neural expression.
8. P{Express-FRT.2.1}
This vector was designed to allow a sequence to be cloned between FRT
sites. The order of the sequences in the vector is
FRT \- miniwhite \- Hsp70 TATA \- polylinker \- alphaTub84B 3'end \- FRT
9. P{Express-FRT.2.2}
This vector was designed to allow the miniwhite marker to be removed after
transformation. The order of the sequences in the vector is
FRT \- miniwhite \- FRT \- Hsp70 TATA \- polylinker \- alphaTub84B 3'end
10. P{Express-FRT.2.3}
This vector is identical to P{Express-FRT.2.2} except for the sequence of
the polylinker, which has more restriction sites.