The following information accompanied stocks donated to the Bloomington Stock Center by Aaron Pilling and Bill Saxton, Indiana University (4/04).
The P{mitoGFP.AP} construct encodes the 31 amino acid mitochondrial import sequence from human cytochrome C oxidase subunit VIII fused to the N terminus of the S65T spectral variant of GFP. The original chimeric protein was described in Rizzuto et al., 1995 (Current Biology 5: 635-642). The chimeric DNA fragment was cloned into pP{UAST}.
P{mitoGFP.AP}2 is a second chromosome insertion.
P{mitoGFP.AP}3 is a homozygous viable and fertile, third chromosome insertion.