Subject: Corrected location for EY01148
I am writing on behalf of the BDGP Gene Disruption Project to correct
an error in the insertion site position for the P-element in the
EY01148 mutant. The FlyBase record has the insertion site in
3R-87B14 associated with the gene Hsp70Bbb. I assume that this site
was based on the insertion site data supplied by our Project, which
mapped it by aligning the flanking sequence with the genome sequence,
probably at a time when only release 2 of the genome sequence was
available. I have reexamined the alignment of the EY01148 flanking
sequence with release 3 of the genome sequence, as well as other
GenBank sequence records (most notably the record with accession
J01103.1  GI:157710 ). The best alignment of the EY01148 flanking
sequence is at AE003693.3 coordinate 207312 (plus strand) = arm_3R
coordinate 7784134, which corresponds to estimated cytogenetic region
87A3-7, between the Hsp70Aa and Hsp70Ab genes. The insertion is 0.16
kb from the annotated 5' end of the Hsp70Ab gene. There is a gap in
the alignment of the EY01148 with the genome sequence in this region
that probably corresponds to a S-element that is annotated in the
BDGP/Celera genome sequence. This S-element appears to be
polymorphic, as it is absent in the genome sequence in GenBank record
J01103. The mapping of the EY01148 flanking sequence was complicated
both by this S-element and by the similarity of the sequences present
upstream of the reiterated copies of the Hsp70 genes.
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Robert W. Levis, Ph.D.
Carnegie Institution of Washington
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