Subject: P{UAS-lacZ.Exel} construct and insertions
The following information was provided by Annette Parks of Exelixis, Inc.
In P{UAS-lacZ.Exel}, lacZ was cloned into EcoR1/Bgll2 sites in the
polylinker of P{Express-UAS}. The lacZ gene was isolated in two fragments:
EcoR1-Sac1 was from pP{C4-AUG-Betagal} (FBmc0000220) and is an in-frame
fusion of the ADH leader to the 5' end of lacZ. The other half of lacZ was
from pDCZA (a clone from Dan Curtis) and has the 3' end of lacZ directly
fused to the ADH 3'UTR immediately following the stop codon.
P{UAS-lacZ.Exel}2 is a homozygous viable and fertile, second chromosome
insertion.
P{UAS-lacZ.Exel}3 is a homozygous viable and fertile, third chromosome
insertion.