Subject: uncoordinated Dear Colleague, We would like to submit an update for the gene CG1501, which we have identified as uncoordinated (unc) (Baker et al., Development 131, 3411-3422). The alleles unc2, unc17 and unc24 have rearrangements within the transcription unit, and the alleles unc9, unc25, unc26 unc27 and unc28 have single-base changes creating stop codons within the ORF. 5RACE was used to extend the sequence from the cDNA clone LP08350 by 538 bases (appended below); the resulting transcript is 164 bp longer than CG1501-RA. The start of the peptide sequence in each sequence is identical as the 164 bp contain 5' untranslated sequence. Given these data the peptide sequences should be identical but an internal deletion of the amino acids 681 VKLRHQAEMLRLSKRQNSWR 700 has made its way into the annotation for the corresponding peptide in BDGP giving rise to a 1366 (as opposed to our predicted 1386 AA) sequence. We suggest that this should be corrected as it is not supported by either the BDGP sequence or our independent sequence for the LP08350 cDNA. We have recently submitted this data to genbank (AY748349). Please let us know if we can provide any more information, Regards, James Baker, Maurice Kernan >unc cDNA (full length) TCACATTTCGCTGTTGGTGGTGAGTGTCTTGCGCTCGTGCTCGTTTTACCTGGAAAGTTCAGTTACTCAAAC GCGGCGCATGTAAGCGGCGGTCAGAGGTTCTATCCGAGCATACGCAGACCAACTCTCGATCCCATCCGAGCC TCGAGTTCCTTCAGGACGGCATGAAGGTTCGCCACGTGCCGGCGAGCACGACAACGTTATCGTTCAGGGCGA AGCAGCGGGATGTGGGCGGCATCGGCATGCCTTGCGAGCGGGAGCGGAAGCACGAGGCCGCAAAGCGGGAGC GGGAGGAGCAGATTAAGCAGGAGCGGGCCACAGCGGCGTCGGCGACACAGCGGGTGCGCCAGCATAATCGAA GGATCAGCACCGACGGCGATCCCGCCAGGCGGAGAATGACGCAGTCCAGGGAGCGACTCCAAGCAACCGGAC TGGGCAGACCAGGACCGTGCGGACCATCAAATATAACTAGAGTAACGCGGGGATCATCATTGCCCCCGGAGG TTGGAGCACACGGATACCGCACAGGAAGGCCTACCAGAAAGCACGTACCTATTCCTCAGGATCAGATTGAGG AGCAGGATGACTCCGATGCCAGGGCGGAACGGCTGAGGCAACAGCTCGAGGAACTGGCCGCGATGTCGGAGC AGGAGTTCGAGGTGAAGTTCCGCCAGTGGCTGGACCAGGAGGGGATTGCCCGGGAGATGCACTCTCACCTGC GGGTGGAGCTCATTCACTGCTTCAATAATACGGCACTCGGCCAGCTCTTGAGCAAGGCTGCTGGTGTGCAGA TGGCTCAGTCCCACGCACTGCTCGTCTCGCCACTGGCGATGGTGCTGCACACTCTGGTGGCGGAGTTTCTGC ACTCCCAGAACTGCCACTTCACACTGTCGGTCTTCTGCAGCGAGACGCCGCATCGCAGCAACCTGCCGGACT TCCGGAGTCGGCCGGAGTTCCGATTCCAGACGGAGGAACTGCAGAAGGTCGTGGCCGCCATACTGGGAGAAA AAGAAGCTCTAGCGGATTCGGAATTTGGAAAGCTGGCGGAGGCCCACTACGAGGAGGATCTGGCTGGACAAA CGCAGTGCTTGCTGATGGCTCTTATGCGAACGCTGGTGGAGATCAGGAGGTCCGTGCCTAAGCAGGAGGTGG AACAACCGGTCCCTGTCTCTCTGCAAGACACCGGCTGTCAAACGGAGCCCTCTGCCTGCGTGGAAGCTCGTC CCGAGGTGGACACCACTGGTCTGTATCAGACGGAGGAGCACGAGCTGATTCTGGGCGCAGATGGCCGAAGTG TCTTTGTGGGCGGTCGTGTCTCGCAATCTCTGCATTCCGTGGAGCAGCAGCTTAATCAGCTGATTAAGAATC TCCGCCATTTGGCCAAGTCGTGTGCTCCGCCCGTTGAGGTGATATCATCGACAAAATTCGAGGATCTCCTAA TGAAGGAGCTGCGCGAAAGGGAGCGACTTTTGAAAGCTGGTCAAGCATTTAACCCGGGCGAACCGGTGATCA AAATTGCCACCGGGGACAAGGAAGAGGAGCATGAGGTAACCAGTGGCAGGAACCAGAAAGATGTGGTTCACT CCAACCTGGGACCCATTCAAGTGCCAGCCCTGGATGTGACCATTCCTCAGCTGCCCCGTCTCCACAGTGAAC AACTGGCCAGCCTGGCCGTGATCCGCCAGTCCCTCGAAAAGGTGCAGAAAAAGAGCCGGCAGCCGCAGGGCC GGATGTACGTCTCCATGGAGCGAATGGAGACCCTGATGGGGGATGTGTGCGGCTGTGTCCAACTACTTAGCA ACGTTCTCAATCTCTCCATGGAGCAGGAGCACTCTGTGGGCATGCACAAGGGCTTCAAATGGGGCTACCGCG AGGGTTTTGCCCACGGCCACTTCATGGGCCTGCAGGAGGGTCAGAAACTGGAGCAGTTGGAACAGAAGAAGT GGCAAAAGGAAAGGCCACTGCCCGAGAAGCGGGAAAGCTCCGTGCAAACGGAGAGCACGATACCCACGGCGA GTATAGCCACCCAAACATCGAGAAAGTCAGCTCACAATGAAAGTGTCATCACCCAAACGGAGCAAAAGCAGC TAAAGGACGCTGGGAATCAGGCTAGTTGCGAGCCGCCAACCTTCCAAAAGTCCTACGAACAGTGGATCCACG AGATGCTGCACAGCTCCAGTGGCCAAGTGTTCCTCGAACGGGTGGAGCTCTCCTTGAACAAGGCGCTGGAGC TGCAAAAGGTGCGGCTAAATGAACTCTTCCAGGTGAAGTTGCGACACCAGGCCGAGATGCTTCGTCTAAGCA AGCGGCAGAACTCCTGGCGGACCCTGTGCAAGCGTGTGGAGCGGGACTCGCAATCGTCAGCGGAGGCGCGCG ACCTCGTGCAGCAGATCTTCCGGCTGCTGGAGCACTACGAGGCACACCATCAGCAGCTGGCGGAGAAGATCC AACAGACGGAGCTTGCAGCGGAGCAGGCGGCTCGCATCATGCCCATGTGGGCGGATGGAGCTGCTGTGGGCT CCGCTTCTGTCAACTGGAACACGGCGATGTCTACCACGCTCATTTCCAGTGGGATCTGCACTCCGCCTCCGG CTCAGCCTGCCGCTGATGGTGCGCTGAATGTTGGTAAGACCCATGTGGAACAGCACACGTATCCTGACCTGG GATATCCCTATCAATTGTTGGCATTAGCTCCGCCAGGTGGAGTTCCAGTTGCAGGCACCTTCGTGCCAGTGA CTCACCTTCCTGCCCACTTGCTTGGGGCTTCTGAACACCCTGTTCCTCTGGTCCCAGTTGCAAGGATCTACA GTGATCCTATGAAAACGTCAAGACGACTGCTTTCTCCGAGTAGCGTTCCTGCAGACAATGACAGTGCTCATC GCGCAGCCGGCGAAACCAATGCTTTCACATTCCAAGGAGGCCCCGTATCTGGGGATCTGCATCCAACTGAAA ATAGAAACCAAGCTCCTGCTACTCAAGGACAACAGTTTCCTGTTCCTTTACCTGTTCCTGCTCCTCGGGGCC CTTCACCAAGTGTACAGAGTGTTTTGCCCTCCACTGCATCACCGGCACTTCCGCCGCCCGATGAAACGGTAC CTCAACCAGGACCTTCCCAATCTTCTATTCCTTTGAAACCGTCAATGCACTCGGTGGCCACCAACACGATAA CTCCAGTGCCTCCAAAGGCGACGCCTCTCAAACCGGTAAATAGCAAAGTTTCAGAAGGTCCGAGCTTTGAGG AGGCTCTACTAAGTGCCAAAAGCCGAATGCTGCAGTTGGAACAGGAGAGCGACCTATTGGAACAAAGTTTCC TCAGCTACCTGGAGAAAACCAAGTCGAAGACATCCCCCCACAAGTCATCGGAGGATGCCGCAAGGGCCCGGA GAATCCGCTCCAGCTGCCTGCGGGAACGAGAGCAGGTGCATCGCACTATGGACGGTTTCCGGGATTGGCATC GACGCGTTCGCAAGGAGGACGCAGCTAGTGTGGCTCAACTGGAGGAGCTGCGCAACCAGCGACTTATACCCG ATCCCATCTTGGACTCAAAGGGGTCGTCACCGCTGTGGCTATCGGAACTGGAGGAGGGTCACGAACAGGACA GCTATCCCTTTACGAATGCCATCACCGAAGCTAGGAACAAGGTGCTGGACAAGATAATGCCAGTCAGAAAGG AGGAGAAGAAGGAGAAGCAAAACGTTAAGAAGAATTGGCTAGCAGATTTGCCATTTCAGATTCCTGAGGCAT CTCCGAAACAGGATCGCCATCTGACCAATGTTAAGCCCGCTGTAACGCGAAGCAGCAGCAATGGTGAGATGA ACGTGCTCCTCCAACGGGCCAAAGATGCCCTCGGCCTGAGAACACCAAACCCACTTCTGGATAGCAGCAGCA GCAGTTCCAGTATGGAGCTGCCCTCCATGGGTGCCGGCAGATCGCCCAGTACAAAGTTCCAGCAATCCATGG CCAGGATGCAGATGCTCTTTGGCGGCGCAGAGGCCGTAAAACCTACAATGCCCGCCAAAACAGGAAGACCTG TGAGTGCACCCACCGCAGCATCGGAACCGGGGTTGTGTGCACATTTGGTGCTCCCGAAACGCCCCCACACGG CTCCCACTCTTCACACAATCAACGAAAATGAACCAGCTGTTCATCATTTGGATGTCTCCACTTCCAGTTCTT CGCAAAGCACTTTGCCCGGGAGATCGTCGGGCGAAGCTTTCGAGGATTTGGTCATGAGCGCCGTGGCGGATG GAGCCCGGCGGCGGAATGCGGAATCTAGCACGGAGGTTTCGTACAGCCAGGCGTTCTGGAAACGCATAAACC TGTAAAAAAATCTAGAATAGGTGCTTAATATGTTCAAACTGATTAAGTATATATCTATTTAGGTTCCAAAAC TCTCTTTATAGAGCAACAAAATAGTAATAATACAATTAATAAAACTCATTGTTTTTTGGCCAAAAAAAAAAA AAAAAAA >UNC_protein MKVRHVPASTTTLSFRAKQRDVGGIGMPCERERKHEAAKREREEQIKQERATAASATQRVRQHNRRISTDGD PARRRMTQSRERLQATGLGRPGPCGPSNITRVTRGSSLPPEVGAHGYRTGRPTRKHVPIPQDQIEEQDDSDA RAERLRQQLEELAAMSEQEFEVKFRQWLDQEGIAREMHSHLRVELIHCFNNTALGQLLSKAAGVQMAQSHAL LVSPLAMVLHTLVAEFLHSQNCHFTLSVFCSETPHRSNLPDFRSRPEFRFQTEELQKVVAAILGEKEALADS EFGKLAEAHYEEDLAGQTQCLLMALMRTLVEIRRSVPKQEVEQPVPVSLQDTGCQTEPSACVEARPEVDTTG LYQTEEHELILGADGRSVFVGGRVSQSLHSVEQQLNQLIKNLRHLAKSCAPPVEVISSTKFEDLLMKELRER ERLLKAGQAFNPGEPVIKIATGDKEEEHEVTSGRNQKDVVHSNLGPIQVPALDVTIPQLPRLHSEQLASLAV IRQSLEKVQKKSRQPQGRMYVSMERMETLMGDVCGCVQLLSNVLNLSMEQEHSVGMHKGFKWGYREGFAHGH FMGLQEGQKLEQLEQKKWQKERPLPEKRESSVQTESTIPTASIATQTSRKSAHNESVITQTEQKQLKDAGNQ ASCEPPTFQKSYEQWIHEMLHSSSGQVFLERVELSLNKALELQKVRLNELFQVKLRHQAEMLRLSKRQNSWR TLCKRVERDSQSSAEARDLVQQIFRLLEHYEAHHQQLAEKIQQTELAAEQAARIMPMWADGAAVGSASVNWN TAMSTTLISSGICTPPPAQPAADGALNVGKTHVEQHTYPDLGYPYQLLALAPPGGVPVAGTFVPVTHLPAHL LGASEHPVPLVPVARIYSDPMKTSRRLLSPSSVPADNDSAHRAAGETNAFTFQGGPVSGDLHPTENRNQAPA TQGQQFPVPLPVPAPRGPSPSVQSVLPSTASPALPPPDETVPQPGPSQSSIPLKPSMHSVATNTITPVPPKA TPLKPVNSKVSEGPSFEEALLSAKSRMLQLEQESDLLEQSFLSYLEKTKSKTSPHKSSEDAARARRIRSSCL REREQVHRTMDGFRDWHRRVRKEDAASVAQLEELRNQRLIPDPILDSKGSSPLWLSELEEGHEQDSYPFTNA ITEARNKVLDKIMPVRKEEKKEKQNVKKNWLADLPFQIPEASPKQDRHLTNVKPAVTRSSSNGEMNVLLQRA KDALGLRTPNPLLDSSSSSSSMELPSMGAGRSPSTKFQQSMARMQMLFGGAEAVKPTMPAKTGRPVSAPTAA SEPGLCAHLVLPKRPHTAPTLHTINENEPAVHHLDVSTSSSSQSTLPGRSSGEAFEDLVMSAVADGARRRNA ESSTEVSYSQAFWKRINL
