Subject: Df(3L)DocA etc molecular information
In Reim et al. (2003) Dev  130:3187-3204  we published
the generation of 3 deficiencies (Fig. 5A), but
exact sequences of breakpoints had not been included
in the article. Here are the molecular data that
characterize the breakpoints of the deficiencies
reported. Data were obtained by sequencing an
inverse PCR product from the 5' end of the retained
P-insertion. The first 60 bases after the P-end are
given (nucleotide 1 which is the first genomic
nucleotide present after the last 5'EP nucleotide
thus characterizes the 1st breakpoint). The
unchanged 3'end of the EP insertion can be
considered the other breakpoint.
Sequence flanking EP(3)3556 in Df(3L)DocA:
CGCTGCACAA TTTAAAATGC CAAATTAGAG TGACCGTCGC
ACGAGGTCGC AAATATGCCA
Sequence flanking EP(3)3556 in Df(3L)DocB:
ACACAATTGA AACATGTCAC ATGCAGTGTG ACCGTTCTTG
GCTCAATGGA AAAGCTCTCA
Sequence flanking EP(3)584 in Df(3L)EP584MR2:
GATACACCAA TGATTTACAA GGGCTGTCAA AGTTTTCAAT
GCACTTAGGA GTGGTCACAC
______________________________________
Ingolf Reim, Ph.D.
Brookdale Department of
Molecular, Cell and Developmental Biology
Mount Sinai School of Medicine
One Gustave L. Levy Place, Box 1020
New York, NY 10029
USA