Nairz, K., Zipperlen, P., Dearolf, C., Basler, K., Hafen, E. (2004). A reverse genetic screen in Drosophila using a deletion-inducing mutagen. Genome Biol. 5(10): R83.
FlyBase ID
FBrf0180325
Publication Type
Research paper
Abstract
We report the use of the cross-linking drug hexamethylphosphoramide (HMPA), which introduces small deletions, as a mutagen suitable for reverse genetics in the model organism Drosophila melanogaster. A compatible mutation-detection method based on resolution of PCR fragment-length polymorphisms on standard DNA sequencers is implemented. As the spectrum of HMPA-induced mutations is similar in a variety of organisms, it should be possible to transfer this mutagenesis and detection procedure to other model systems.
