Subject: incorrect link?
Hello Kathy, and hello to whoever picks this up at Flybase:
Just to let you know:
We obtained 2 lines which BLAST suggested should be in the same gene-
CG3450
==========
The first line is correct, i.e. BL \#10528 IS in CG3450, via our inverse PCR.
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The second of these lines was labeled at BL as not complementing an allele
of wunen (a different gene):
BL-11203 y1 w67c23; P{w+mC=lacW}l(2)k16806k16806/CyO
l(2)k16806
but the BLAST with its accession number:
AQ026117
said it should be in CG3450:
http://flybase.net/cgi-bin/gbrowse_fb/dmel?name= 2R:2322147..2322270 ;ft=blast,Bla
st,,2322147..2322270;doexpand=1
The genetics is right- the hit IS in wunen (I can send our inverse PCR
sequences if you wish), but the blast with the above sequence accession,
which I just re-BLASTed, must be incorrect somehow- or there are 2 P's in
the stock. The latter possibility isn't likely, at least for the recessive
lethality, since the two above lines complement.
I thought you might like to know.
Best,
Lenny
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Leonard Rabinow
Signalisation, Developpement et Cancer
Batiment 442 bis
Universite Paris XI
91405 ORSAY CEDEX
FRANCE