Personal communication to FlyBase from Rajendra K Thimmappaiaha 4 Feb
2005
Subject: CG8069 sequence annotation
This is about the annotation for the gene CG8069. The database shows
two transcripts for this gene, CG8069-RA and CG8069-RB.
Though I don't know how the putative translation products were
predicted, but it appears from sequence alignments of PHAX from various
other species that the real translation start for CG8069 is \+86bp in
CG8069-RA
(http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=24651981).
This is further corroborated by a search for translation start
sequences: \+86 of RA and \+74 of RB (5'-ATGATGGAAC-3') have the genuine
translation start sites as the upstream of these starts (AAAA) match
the consensus sequence (C/AAAA/C) for Drosophila translation start site
(Cavener, 1987). Taking these two aspects into consideration, only
8069-RA can produce a full length protein (starting with \+86bp,
5'-ATGATGGAAC-3') as CG8069-RB is full of stops.