Subject: Re: Fwd: FlyBase Query (cy2593) Dear Chihiro, here is the requested info. the mir-2b construct is based on miR-2b-2 (the miRNA in the spitz intron). the miR-6 construct was obtained from Eric Lai as indicated in the paper and it contains all miR-6 hairpins as they are clustered in the genome. i'll be happy to answer any further questions. best, julius >> Dear Dr Cohen, >> >> I am currently curating your paper for FlyBase: >> >> Brennecke et al., 2004, PLoS Biol. 3(3): 404--418 >> >> I have a couple of quick questions I was hoping you could answer for me: >> >> mir-2b >> ====== >> You make a UAS construct using mir-2b sequences. However we have two >> genes in our records mir-2b-1, mir-2b-2. Could you tell me which one of >> these you used to make your construct? >> >> mir-2b-1 >> CR32990 >> 29A1 >> AE003620 >> AJ421759 >> MIR:MI0000119 >> >> mir-2b-2 >> CR32964 >> 37F2 >> AE003663 >> AJ421760 >> MIR:MI0000120 >> >> mir-6 >> ====== >> Likewise with mir-6. You make a UAS construct using mir-2b sequences. >> However we have three genes in our records that this could be. Could >> you tell me which one of these you used to make your construct? >> >> mir-6-1 >> CR33004 >> 56E1 >> AE003795 >> AJ421764 >> MIR:MI0000124 >> Rfam:RF00143 >> >> mir-6-2 >> CR33040 >> 56E1 >> AE003795 >> AJ421765 >> MIR:MI0000125 >> Rfam:RF00143 >> >> mir-6-3 >> CR33039 >> 56E1 >> AE003795 >> AJ421766 >> MIR:MI0000126 >> Rfam:RF00143 >> >> Best wishes, >> >> Chihiro
