Isolation and characterization of Df(2L)BSC110
Jennifer Deal and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2L)BSC110 was isolated as a FLP recombinase-induced recombination event involving P{XP}d06631 and PBac{RB}e04284. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; P{XP}d06631/PBac{RB}e04284 males crossed to w1118; P{w+mC=hs-hid}2, wgSp-1/CyO females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et. al, Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.RB3}BSC110 from the segment of P{XP}d06631 to the left of its FRT site and the segment of PBac{RB}d04284 to the right of its FRT site. Exelixis, Inc. determined the insertion site of P{XP}d06631 to be at Release 3 genomic coordinate 5022167 on chromosome arm 2L and the insertion site of PBac{RB}e04284 to be at Release 3 genomic coordinate 5057196 on arm 2L. The Gene Disruption Project determined the insertion site of P{XP}d06631 to be at Release 3 genomic coordinate 5022166 on arm 2L and the insertion site of PBac{RB}e04284 to be at Release 3 genomic coordinate 5057191 on arm 2L. The cytological breakpoints of Df(2L)BSC110 predicted from these coordinates are 25C1;25C3. It failed to complement Cg25Ck00405, eIF-3p40k09003 and Df(2L)BSC51.