Isolation and characterization of Df(3L)BSC123
Rachel Andrade and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3L)BSC123 was isolated as a FLP recombinase-induced recombination event involving P{XP}d03930 and PBac{WH}f05230. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, w1118; P{XP}d03930/PBac{WH}f05230 females crossed to w1118; P{w+mC=hs-hid}2, wgSp-1/CyO males. These females were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al, Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC123 from the segment of P{XP}d03930 to the left of its FRT site and the segment of PBac{WH}f05230 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. Exelixis, Inc. determined the insertion site of P{XP}d03930 to be at Release 3 genomic coordinate 1775286 on chromosome arm 3L and the insertion site of PBac{WH}f05230 to be at Release 3 genomic coordinate 1786783 on arm 3L. The Gene Disruption Project determined the insertion site of P{XP}d03930 to be at Release 3 genomic coordinate 1775476 on arm 3L and the insertion site of PBac{WH}f05230 to be at Release 3 genomic coordinate 1786781 on arm 3L. The cytological breakpoints of Df(3L)BSC123 predicted from these coordinates are 62B4;62B4. It failed to complement Cdc37e4D and Df(3L)Exel6088.