Isolation and characterization of Df(3L)BSC128
Rachel Andrade, Jill Gresens, Megan Deal and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3L)BSC128 was isolated as a FLP recombinase-induced recombination event involving PBac{RB}Mtche00205 and P{XP}thoc7d05792. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118, P{hsFLP}1; PBac{RB}Mtche00205/P{XP}thoc7d05792 females crossed to w1118; wgSp-1/CyO; sensLy-1/TM6B, Tb1 males. The females were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.RB3}BSC128 from the segment of PBac{RB}Mtche00205 to the left of its FRT site and the segment of P{XP}thoc7d05792 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al with the substitution of the primer 5'-CCAATGCGTTTATTTCAGGTCACG-3' for the RB3' minus primer in the Hybrid PCR protocol in the Supplementary Methods. The cytological breakpoints of Df(3L)BSC128 predicted from the transposable element insertions sites are 61B2;61B2. It failed to complement Df(3L)Exel6084.