Isolation and characterization of Df(3L)BSC129
Rachel Andrade, Jill Gresens, Megan Deal and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3L)BSC129 was isolated as a FLP recombinase-induced recombination event involving P{XP}d01788 and PBac{WH}CG12077f05249. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118, P{hsFLP}1; P{XP}d01788/ PBac{WH}CG12077f05249 females crossed to w1118; wgSp-1/CyO; sensLy-1/TM6B, Tb1 males. The females were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC129 from the segment of P{XP}d01788 to the left of its FRT site and the segment of PBac{WH}CG12077f05249 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. Exelixis, Inc. determined the insertion site of P{XP}d01788 to be at Release 3 genomic coordinate 3227264 on arm 3L. The cytological breakpoints of Df(3L)BSC129 predicted from the transposable element insertions sites are 63C1;63D1. It failed to complement Df(3L)1227, Df(3L)HR232 and Df(3L)HR119.