Isolation and characterization of Df(3L)BSC130
Rachel Andrade, Jill Gresens, Megan Deal and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3L)BSC130 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}CG5660f01783 and P{XP}bold01760. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118, P{hsFLP}1; PBac{WH}CG5660f01783/P{XP}bold01760 females crossed to w1118; wgSp-1/CyO; sensLy-1/TM6B, Tb1 males. The females were heat shocked as larvae as described in Parks et al. Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC130 from the segment of PBac{WH}CG5660f01783 to the left of its FRT site and the segment of P{XP}bold01760 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. Polytene chromosome squashes of Df(3L)BSC130 showed the breakpoints 66E5-F1;66F5-67A1.