Isolation and characterization of Df(3R)BSC124
Rachel Andrade, Jill Gresens, Megan Deal and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3R)BSC124 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}f01767 and P{XP}CG17838d09234. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118, P{hsFLP}1; PBac{WH}f01767/P{XP}CG17838d09234 males crossed to w1118; P{hs-hid}3, Dr1/TM6B females. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC124 from the segment of PBac{WH}f01767 to the left of its FRT site and the segment of P{XP}CG17838d09234 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(3L)BSC124 predicted from the transposable element insertions sites are 92F10;92F12-93A1. It failed to complement cdc2c3 and P{PZ}CG1783803806.