Subject: Re: Fwd: Question about Oseg1 alleles Dear Beverley, You are correct in Figure 4D Oseg1179 should be E243. Tomer >>Subject: Question about Oseg1 alleles >> >>Dear Dr. Zuker, >> >>I am curating the positions of the Oseg alleles reported in your >>Avidor-Reiss et al., 2004, Cell 117(4): 527--539 paper to >>appear in the genome maps in FlyBase. >>I found an inconsistency in the reported location for Oseg1179 >>in Figure 4. >> >>Figure 4a gives the nucleotide substitutions for the Oseg1 mutations as >> >>Oseg1179 \- G881A >>Oseg110 \- C2279T >> >>While Figure 4D lists the amino acid substitutions as >> >>Oseg1179 \- E727stop >>Oseg110 \- Q689stop >> >>These statements are not consistent with each other and >>the amino acid statement E727stop is not consistent with >>the position of Oesg1 shown on the maps of transcript and >>protein. >> >>I was hoping you could clear this up for me. >>Calculating back from the reported nucleotide position >>of Oseg1179, I am guessing the mutation is at E243. >>Please let me know what the actual position of the mutation >>is. Thanks for your help, >> >>Sincerley, >> >>Beverley Matthews >>FlyBase-Harvard > >-- >Charles S. Zuker >Professor of Biology and Neurosciences >Investigator, Howard Hughes Medical Institute >UCSD >9500 Gilman Drive, CMMW 355 >La Jolla, CA 92093-0649 \-- Tomer Avidor-Reiss, Ph.D. Harvard Medical School Department of Cell Biology 250 Longwood Ave. Boston MA 02115 Seeley G. Mudd Bldg, Room 509b
