Isolation and characterization of Df(2L)BSC149
Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2L)BSC149 was isolated as a FLP recombinase-induced recombination event involving P{XP}d04716 and PBac{WH}CG31791f04849. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; P{XP}d04716/PBac{WH}CG31791f04849 males crossed to w1118; P{hs-hid}2, wgSp-1/CyO females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC149 from the segment of P{XP}d04716 to the left of its FRT site and the segment of PBac{WH}CG31791f04849 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. Exelixis, Inc. determined the insertion site of P{XP}d04716 to be at Release 3 genomic coordinate 18422773 on chromosome arm 2L. The Gene Disruption project determined the insertion site of P{XP}d04716 to be at Release 3 genomic coordinate 18422810 on arm 2L. The cytological breakpoints of Df(2L)BSC149 predicted from the transposable element insertions sites using release 3 coordinates are 36E3;36E10. It failed to complement Df(2L)Exel8038 and Df(2L)Exel6041.