Isolation and characterization of Df(2R)BSC153
Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2R)BSC153 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}Roc2f00911 and P{XP}d01597.  The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; PBac{WH}Roc2f00911/P{XP}d01597 males crossed to w1118; P{hs-hid}2, wgSp-1/CyO females.  These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003).  This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002).  The recombination event generated the genetic element P+PBac{XP5.WH5}BSC153 from the segment of PBac{WH}Roc2f00911 to the left of its FRT site and the segment of P{XP}d01597 to the right of its FRT site.  Its presence was verified using the PCR methods and primers described in Parks et al.  The cytological breakpoints of Df(2R)BSC153 predicted from the transposable element insertions sites using Release 4 coordinates are 48B4-C1;48D7.  It failed to complement wal02516.